2011 Fiscal Year Final Research Report
Investigation of a cytosolic sulfur transfer pathway for tRNA thio-modification and biosynthesis of sulfur-containing molecules
| Project/Area Number |
21570150
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Osaka Medical College |
|---|
Principal Investigator |
NAKAI Yumi 大阪医科大学, 医学部, 講師 (80268193)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | tRNA硫黄修飾 / 酵母 / ミトコンドリア / サイトゾル / 鉄硫黄クラスター / システインデスルフラーゼ |
|---|
| Research Abstract |
We found that ubiquitin-like protein Urm1 and ubiquitin-activating enzyme-like protein Uba4 were strictly required for the s2 modification. The carboxyl-terminal glycine residue of Urm1 was critical for the s2 modification, indicating direct involvement of the unique ubiquitin-related system in this process. We also demonstrated that the s2 and mcm5 modifications in cytosolic tRNAs influence each other's efficiency. Taken together, our data indicate that the s2 modification of cytosolic tRNAs is a more complex process that requires additional unidentified components.
|
