2011 Fiscal Year Final Research Report
Bidirectional evolution of an ancestral gene for sex determination : testis and ovary determination
| Project/Area Number |
21570239
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
MIURA Ikuo 広島大学, 大学院・理学研究科, 准教授 (10173973)
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| Co-Investigator(Renkei-kenkyūsha) |
OHTANI Hiromi 広島大学, 大学院・理学研究科, 助教 (20106800)
ICHIKAWA Youko 県立広島大学, 生命環境学部, 准教授 (20084163)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 性決定 / SRY / 卵巣決定 / SOX3 |
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| Research Abstract |
We examined the function of the sex-linked SOX3 for ovarian differentiation in the frog Rana rugosa. SOX3 is known to be an ancestral gene of the male determining gene SRY in eutherian mammals. Short nucleotide elements specific to the upstream regions of W-SOX3 and Z-SOX3, respectively, were identified. In the undifferentiated gonads of ZW, W-SOX3 was highly expressed and the higher expression preceded the onset of sexually dimorphic expression of CYP19 and FOXL2. Using transgenesis, we successfully obtained ZZ frogs of which germ cells were introduced by the W-SOX3, but their gonads did not differentiate ovaries.
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Research Products
(28 results)
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[Presentation] The W chromosome evolutionand sex-linked gene expression in theJapanese frog Rana rugosa2009
Author(s)
Miura, I., Ezaz, T., Ohtani, H., Uno, Y., Nishida, N., Matsuda, Y., and Graves, JAM.
Organizer
Fifthinternational syumposium on thebiology of vertebrate sexdetermination
Place of Presentation
Kona, Hawaii, USA
Year and Date
20090420-24
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