2011 Fiscal Year Final Research Report
Identification of polyembryogenesis regulation gene in Citrus and diversification.
| Project/Area Number |
21580005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Shizuoka University |
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Principal Investigator |
OMURA Mitsuo 静岡大学, 農学部, 教授 (90355397)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 遺伝子 / ゲノム / 育種 / 発生・分化 |
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| Research Abstract |
To understand the molecular mechanism of apomixis in plant, the positional cloning method was applied to identify the gene regulating nucellar embryogenesis in Citrus. The 380 kb covering the locus responsible to somatic embryogenesis on the linkage group 1 was sequenced and the diversification of the region was surveyed. The ORFs detected in the region were compared in their structure and expression between polyembryony and monoembryony haplotypes. The genes expressing during the somatic embryogenesis from cultured callus were also explored to associate with the responsible gene in nucellar embryogenesis.
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[Journal Article] PCR primer for marker assisted backcrossing to introduce a CTV resistance gene from Poncirus trifoliate(L.) Raf. into Citurs.2011
Author(s)
Ohta, S., T. Endo, T. Shimada, H. Fujii, T. Shimizu, T. Kuniga, T. Yoshioka, H. Nesumi, T. Yoshida, and M. Omura
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Journal Title
J. Japan. Soc. Hort. Sci
Volume: 80
Pages: 295-307
Peer Reviewed
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