2011 Fiscal Year Final Research Report
Construction of recombinant baculoviruses without using cell cultures
| Project/Area Number |
21580064
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
NAKAI Madoka 東京農工大学, 大学院・農学研究院, 准教授 (60302907)
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| Co-Investigator(Renkei-kenkyūsha) |
KANG Wonkyon 理化学研究所, 本分子昆虫学研究室, 専任研究員 (30291917)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 組換えウイルス / 顆粒病ウイルス / 核多角体病ウイルス / トランスファーベクター / チャノコカクモンハマキ / バックミド |
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| Research Abstract |
Construction of recombinant viruses is important for elucidating viral gene function and infection processes, as well as for recombinant protein production. However, with the exception of a few model systems, most of the～600 known baculoviruses lack permissive cell lines in which recombinant genotypes can be produced and, consequently, the molecular pathology of those viruses is poorly understood. The purpose of this study was to establish a generally applicable methodology to construct recombinant baculoviruses without having to use cell culture. In this study, the bacmid system was constructed using baculoviruses genome introduced with bacterial replication origin and generated recombinant virus DNA was established.
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