2011 Fiscal Year Final Research Report
Analysis of substrate-recognition mode by novel ubiquitin ligase related to endoplasmic reticulum-associated degradation pathway.
| Project/Area Number |
21580121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | 財団法人東京都医学総合研究所 (2011)
Tokyo Metropolitan Organization for Medical Research (2009-2010) |
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Principal Investigator |
YOSHIDA Yukiko 財団法人東京都医学総合研究所, 生体分子先端研究分野, 主任研究員 (90271543)
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| Project Period (FY) |
2009 – 2011
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| Keywords | F-box蛋白質 / ユビキチンリガーゼ / 小胞体関連分解 / N型糖鎖 / 基質認識 |
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| Research Abstract |
We have reported previously that Fbs1 and Fbs2 are glycoprotein-specific F-box proteins, which are identified as the endoplasmic reticulum-associated degradation(ERAD)-linked ubiquitin ligase component. Here we report that Fbxo44b, which does not have sugar-binding activity, is involved in ubiquitylation of glycoproteins under the ERAD pathway. In addition, we analyzed the third F-box protein recognizing N-glycoproteins, Fbs3.Fbs3 can interact with glycoproteins modified with not only high-mannose but also complex-type glycans, suggesting that participates in unknown function of glycoproteins endocytosed from the cell surface but not through the ERAD pathway.
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