2011 Fiscal Year Final Research Report
Molecular pathological study on the mechanism of epilepsy in familial epileptic dog
| Project/Area Number |
21580373
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | イヌ / 遺伝 / グルタミン酸 / グルタミン酸トランスポーター / てんかん / 脳 |
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| Research Abstract |
Prior study in this familial epileptic Shetland sheepdog has suggested that a decrease of GLT-1 expression in astrocytes in the cortex under the sulcus may be responsible for the occurrence of abnormal electroencephalographic waves. In this study, we investigated where the process of GLT-1 synthesis would be affected in astrocytes. Immunohistochemically, a decrease of GLT-1 immunolabellings was found in the cerebral cortex under the sulcus. In situ hybridization demonstrated no significant changes of GLT-1 mRNA labellings in the cortex under the sulcus of all familial dogs. In immunoelectron microscopy, GLT-1 immunolabellings were detected in endoplasmic reticulum(ER), while not the plasma membrane of astrocytes in the familial cortex under the sulcus. These results suggested at least two possibilities that GLT-1 synthesis process may stop in ER following an incomplete translation, or that the post-translational process of GLT-1 synthesis may be affected.
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