Analysis of myosin sliding on wide variety of actin-rails.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590270
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Gunma University
• Principal Investigator: ISHIKAWA Ryoki 群馬大学, 大学院・医学系研究科, 講師 (20212863)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Akio 群馬大学, 大学院・医学系研究科, 講師 (30282388) ; KOHAMA Kazuhiro 群馬大学, 大学院・医学系研究科, 教授 (30101116)
• Project Period (FY): 2009 – 2011
• Keywords: アクチン / ミオシン

Research Abstract

To examine whether or not brain-specific actin binding protein drebrin affects the activity of myosin-V, we observed the sliding of myosin-V on F-actin in the presence or absence of drebrin. We found that myosin-V slides on drebrin-decorated F-actin as fast as "naked" F-actin, whereas sliding distance and attachment time were smaller in the presence of drebrin than that in the absence of drebrin. Drebrin inhibits the binding of leading head to F-actin, but does not inhibit the release of ADP from trailing head. These results suggest that drebrin modulates the affinity of myosin-V to F-actin, thus inhibits the activity of myosin-V, and affects the motility of the central zone of nerve cell growth cones.

Research Products

• [Journal Article] Down regulation of myosin light chain kinase expression in vascular smooth muscle cells accelerates cell proliferation : Requirement of its actin-binding domain for reversion to normal rates (2012)
  • Author(s): Wang HH, Nakamura A, Yoshiyama S, Ishikawa R, Cai N, Ye LH, Takano-Ohmuro H, Kohama K
  • Journal Title: J. Pharmacol. Sci Volume: (in press)
  • DOI: DOI:10.1254/jphs.11213SC
  • Peer Reviewed
• [Journal Article] Video imaging of walking myosin V by high-speed atomic force microscopy (2010)
  • Author(s): Kodera N, Yamamoto D, Ishikawa R, Ando T
  • Journal Title: Nature Volume: 468 Pages: 72-76
  • DOI: DOI:10.1038/nature09450
  • Peer Reviewed
• [Journal Article] Modulation of the mechano-chemical properties of myosin V by drebrin-E (2010)
  • Author(s): Kubota H, Ishikawa R, Ohki T, Ishizuka J, Mikhailenko SV, Ishiwata S
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 400 Pages: 643-648
  • DOI: DOI:10.1016/j.bbrc.2010.08.120
  • Peer Reviewed
• [Journal Article] Utilization of myosin and actin bundles for the transport of molecular cargo (2010)
  • Author(s): Takatsuki H, Rice KM, Asano S, Day BS, Hino M, Oiwa K, Ishikawa R, Hiratsuka Y, Uyeda TQ, Kohama K, Blough ER
  • Journal Title: Small Volume: 6 Pages: 452-457
  • DOI: DOI:10.1002/small.200901369
  • Peer Reviewed
• [Journal Article] Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain (2010)
  • Author(s): Katayama K, Watanabe M, Tanaka H, Hino M, Miyakawa T, Ohki T, Ye LH, Xie C, Yoshiyama S, Nakamura A, Ishikawa R, Tanokura M, Oiwa K, Kohama K
  • Journal Title: Am. J. Physiol. (Heart Cir. Physiol.) Volume: 298 Pages: H505-514
  • DOI: DOI:10.1152/ajpheart.00577.2009
  • Peer Reviewed
• [Journal Article] Calcium regulation of non-kinase and kinase activities of recombinant myosin light-chain kinase and its mutants (2009)
  • Author(s): Xie C, Zhang Y, Wang HH, Matsumoto A, Nakamura A, Ishikawa R, Yoshiyama S., Hayakawa K, Kohama K, Gao Y
  • Journal Title: IUBMB Life Volume: 61 Pages: 1092-1098
  • DOI: DOI:10.1002/iub.266
  • Peer Reviewed
• [Journal Article] Nonkinase activity of MLCK in elongated filopodia formation and chemotaxis of vascular smooth muscle cells toward sphingosylphosphorylcholine (2009)
  • Author(s): Wang HH, Nakamura A, Matsumoto A, Yoshiyama S, Qin X, Xie C, Zhang Y, Gao Y, Ishikawa R, Kohama K
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: 296 Pages: H1683-1693
  • DOI: DOI:10.1152/ajpheart.00965.2008
  • Peer Reviewed
• [Presentation] The novel calmodulin-binding site of the smooth muscle myosin light chain kinase (2010)
  • Author(s): Nakamura A, Matsumoto A, Xie C, Yoshiyama S, Ishikawa R, Kohama K
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 20100000
• [Presentation] Dynamics of fascin, cofilin, and drebrin in growth cones : Comparison with the results obtained by in vitro analysis (2009)
  • Author(s): Ishikawa R
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 20090000
• [Presentation] 成長円錐におけるドレブリンとコフィリンの役割 (2009)
  • Author(s): 石川良樹, 加藤薫, 五十嵐道弘, 小浜一弘
  • Organizer: 神経組織の成長・再生・移植研究集会第24回学術集会
  • Place of Presentation: 伊香保
  • Year and Date: 20090000
• [Patent(Industrial Property Rights)] 束化アクチンを用いたマイクロアクチュエータ (2011)
  • Inventor(s): 日野瑞城, 小濱一弘, 石川良樹, 中村彰男, 須齋嵩, 大畠昭子, 大岩和弘
  • Industrial Property Rights Holder: 国立大学法人群馬大学、独立行政法人情報通信研究機構
  • Industrial Property Number: 特許、第4799007号
  • Acquisition Date: 2011-08-12