Establishment and evaluation of the measurement of serum Smad1 in diabetic vascular complications.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21590628
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kagawa University (2011); The University of Tokushima (2009-2010)
• Principal Investigator: KONDO Naoki 香川大学, 医学部附属病院, 病院助教 (80522789)
• Co-Investigator(Kenkyū-buntansha): TOMINAGA Tatsuya 徳島大学, 大学院・ヘルスバイオサイエンス研究科, 助教 (80425446) ; MATSUURA Motokazu 徳島大学, 大学院・ヘルスバイオサイエンス研究科, 助教 (10403734)
• Project Period (FY): 2009 – 2011
• Keywords: 糖尿病 / 血管合併症 / Smad1 / バイオマーカー / BMP4 / MGP

Research Abstract

In order to clarify the functional role of Smad1 in vascular cells during the course of progression of diabetic vascular complications, potential of serum Smad1 as an early biomarker to detect the development and progression of atherosclerosis in type 2 diabetes was examined.(1) Existence of non-phosphorylated, C-terminus phosphorylated and linker-phosphorylated Smad1 was found. Profiles for the values of each Smad1 form in serum were made.(2) Serum MGP was found as a surrogate marker for evaluation of motions of Smad1-related molecules in STZ-induced diabetic mice models.(3) Negative association was observed between severity of atherosclerosis in abdominal aorta and serum MGP levels in patients. Significant difference of MGP-SNPs in the influence on the development of diabetic vascular complications was found, which are independent of the levels of serum MGP in these patients.

Research Products

• [Journal Article] Activation of bone morphogenetic protein 4 signaling leads to glomerulosclerosis that mimics diabetic nephropathy (2011)
  • Author(s): Tominaga T, Abe H, Ueda O, Goto C, Nakahara K, Murakami T, Mima A, Nagai K, Araoka T, Kishi S, Fukushima N, Jishage KI, Doi T
  • Journal Title: J Biol Chem Volume: 286(22) Pages: 20109-16
  • DOI: doi:10.1074/jbc.M110.179382
  • Peer Reviewed
• [Journal Article] BMP4・Smad1シグナルと糖尿病性腎症 (2011)
  • Author(s): 安部秀斉、冨永辰也、松原雄、土井俊夫
  • Journal Title: 日本腎臓学会誌 Volume: vol.53, No.7 Pages: 1000-1005
• [Presentation] 尿中エクソゾームを用いた慢性腎臓病の非侵襲的診断法と予後予測 (2011)
  • Author(s): 林早苗、安部秀斉、冨永辰也、安部尚子、近藤直樹、土井俊夫
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市
  • Year and Date: 20111113-16
• [Presentation] Genomic biomarkerを用いたCKD患者の心血管リスク評価の試み (2011)
  • Author(s): 吉川和寛、安部秀斉、冨永辰也、岸誠司、近藤直樹、松浦元一、長井幸二郎、土井俊夫、中村雅将、土田健司、水口潤、川島周
  • Organizer: 第56回日本透析医学会学術集会・総会
  • Place of Presentation: 横浜
  • Year and Date: 20110617-19
• [Presentation] 新規バイオマーカーとしてのpodocyte由来尿中エクソゾームによる慢性腎臓病患者の病態解析 (2010)
  • Author(s): 安部尚子、安部秀斉、近藤直樹、吉川和寛、土井俊夫
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 20101207-10
• [Presentation] CKD患者の血中MGPおよび関連分子と、病態に関する検討 (2010)
  • Author(s): 吉川和寛、安部秀斉、冨永辰也、岸誠司、岸史、荒岡利和、村上太一、近藤直樹、松浦元一、長井幸二郎、土井俊夫、中村雅将、土田健司、水口潤、川島周
  • Organizer: 第55回日本透析医学会学術集会・総会
  • Place of Presentation: 神戸
  • Year and Date: 20100618-20
• [Presentation] 新規バイオマーカーとしての尿中エクソゾーム中のCD2APによるCKD患者の予後解析 (2010)
  • Author(s): 近藤直樹、安部秀斉、芦名茂人、吉川和寛、岸史、荒岡利和、岸誠司、松浦元一、村上太一、中村雅将、長井幸二郎、土井俊夫
  • Organizer: 第55回日本透析医学会学術集会・総会
  • Place of Presentation: 神戸市
  • Year and Date: 20100618-20
• [Presentation] BMP4/ Smad1 Signaling Is a Critical Therapeutic Target for Diabetic Nephropathy (2010)
  • Author(s): Matsubara T, Abe H, Ueda O, Tominaga T, Mima A, Nagai K, Torikoshi K, Araki M, Goto C, Kinosaki M, Jishage K, Fukushima N, Iehara N, Fukatsu A, Arai H, Doi T
  • Organizer: 43^<rd> American Society of Nephrology(ASN) Annual
  • Place of Presentation: Meeting, Denver
  • Year and Date: 2010-11-19
• [Presentation] 加齢による影響を受けない腎症新規バイオマーカーの探索 (2010)
  • Author(s): 芦名茂人、安部秀斉、土井俊夫
  • Organizer: 第21回日本老年医学会四国地方会総会
  • Place of Presentation: 徳島大学医学部・歯学部歯学部附属病院(徳島市)
  • Year and Date: 2010-02-20
• [Presentation] 糖尿病性腎症発症とアルブミン尿排泄に関与するBMP4の分子機構
  • Author(s): 冨永辰也
  • Organizer: 第52回日本生化学会中国・四国支部総会
  • Place of Presentation: 広島大学・広仁会館(広島市)