Cell biological analysis of the effect of hypoxia-induced gene expressions on progression of pulmonary alveolar inflammation

2011 Fiscal Year Final Research Report

• Project/Area Number: 21591992
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: 公益財団法人田附興風会 (2011); Tazuke Kofukai Medical Research Institute (2009-2010)
• Principal Investigator: ADACHI Takehiko 公益財団法人田附興風会, 医学研究所第9研究部, 部長 (90252428)
• Co-Investigator(Kenkyū-buntansha): HIROTA Kiichi 京都大学, 医学研究科, 准教授 (00283606)
• Project Period (FY): 2009 – 2011
• Keywords: 肺胞上皮 / 肺障害 / 低酸素誘導性因子1 / タバコ / 水チャネル / 遺伝子発現

Research Abstract

Systemic shock manifested as a symptom of sepsis and tabaco smoking disturb physiological homeostasis and induces phothophysiological changes in epithelium, interstitial tissue and capillary vessels of pulmonary alveoli as a unit of gas exchange. To review these pathological phenomena from the point of signal transduction and gene expression elicited by systemic and tissue hypoxia and understand molecular mechanisms behind the phenomena, we analysed cells and mice adopting methodology of molecular biology in relation to hypoxia-inducible factor 1 activity.

Research Products

• [Journal Article] Hydrogen sulfide inhibits hypoxia-but not anoxia-induced hypoxia-inducible factor 1 activation in a von hippel-lindau-and mitochondria-dependent manner (2012)
  • Author(s): Kai, S., Tanaka, T., Daijo, H., Harada, H., Kishimoto, S., Suzuki, K., Takabuchi, S., Takenaga, K., Fukuda, K., and Hirota, K.
  • Journal Title: Antioxidants & redox signaling Volume: 16 Pages: 203-216
  • DOI: DOI:10.1089/ars.2011.3882
  • Peer Reviewed
• [Journal Article] General Anesthetics Inhibit Erythropoietin Induction under Hypoxic Conditions in the Mouse Brain (2011)
  • Author(s): Tanaka, T., Kai, S., Koyama, T., Daijo, H., Adachi, T., Fukuda, K., and Hirota, K.
  • Journal Title: PLoS ONE Volume: 6 Pages: e29378
  • DOI: DOI:10.1371/journal.pone.0029378
  • Peer Reviewed
• [Journal Article] Fentanyl activates hypoxia-inducible factor 1 in neuronal SH-SY5Y cells and mice under non-hypoxic conditions in a mu-opioid receptor-dependent manner (2011)
  • Author(s): Daijo, H., Kai, S., Tanaka, T., Wakamatsu, T., Kishimoto, S., Suzuki, K., Harada, H., Takabuchi, S., Adachi, T., Fukuda, K., Hirota, K.
  • Journal Title: European journal of pharmacology Volume: 667 Pages: 144-152
  • DOI: DOI:10.1016/j.ejphar.2011.06.014
  • Peer Reviewed
• [Journal Article] 炎症の制御点としての低酸素誘導性因子 (2011)
  • Author(s): 広田喜一,大条鉱樹,甲斐慎一,鈴木堅悟,岸本俊,松山智紀
  • Journal Title: 麻酔 Volume: 60 Pages: S69-74
  • URL: http://jglobal.jst.go.jp/public/20090422/201102269918990320
• [Journal Article] Persisting mild hypothermia suppresses hypoxia-inducible factor-1alpha protein synthesis and hypoxia-inducible factor-1-mediated gene expression (2010)
  • Author(s): Tanaka, T., Wakamatsu, T., Daijo, H., Oda, S., Kai, S., Adachi, T., Kizaka-Kondoh, S., Fukuda, K., and Hirota, K.
  • Journal Title: American journal of physiology Regulatory, integrative and comparative physiology Volume: 298 Pages: R661-671
  • DOI: DOI:10.1152/ajpregu.00732.2009.
  • Peer Reviewed
• [Journal Article] The calcium channel blocker cilnidipine selectively suppresses hypoxia-inducible factor 1 activity in vascular cells (2009)
  • Author(s): Oda, S., Oda, T., Takabuchi, S., Nishi, K., Wakamatsu, T., Tanaka, T., Adachi, T., Fukuda, K., Nohara, R., and Hirota, K.
  • Journal Title: Eur J Pharmacol Volume: 606 Pages: 130-136
  • DOI: DOI:10.1016/j.ejphar.2009.01.012
  • Peer Reviewed
• [Journal Article] ハイポキシア生物学-酸素代謝から見る生命現象- (2009)
  • Author(s): 広田喜一,若松拓彦,田中具治,大条鉱樹,甲斐慎一
  • Journal Title: 麻酔 Volume: 58 Pages: S138-S146
• [Presentation] Isoflurane selectively inhibits the activation of hypoxia-inducible factor 2 and the induction of erythropoietin under hypoxic conditions in mouse brain (2011)
  • Author(s): Kiichi Hirota, Tomoharu Tanaka, Shinichi Kai, Tomohiro Koyama, Hiroki Daijo, Kengo Suzuki, Takehiko Adachi, Kazuhiko Fukuda
  • Organizer: 2011 Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-16
• [Presentation] 炎症の制御点としての低酸素誘導性因子 (2011)
  • Author(s): 広田喜一、大条鉱樹、甲斐慎一、鈴木堅悟,岸本俊、松山智紀
  • Organizer: 日本麻酔科学会第58回学術総会
  • Place of Presentation: 神戸市
  • Year and Date: 2011-05-20
• [Presentation] タバコ煙抽出物はヒト肺胞 & 型上皮細胞株(A549)において低酸素誘導性因子1(hypoxia-inducible factor 1 : HIF-1)を活性化する (2011)
  • Author(s): 大条紘樹、鈴木堅固,岸本俊、甲斐慎一、広田喜一、福田和彦
  • Organizer: 日本麻酔科学会第58回学術総会
  • Place of Presentation: 神戸市
  • Year and Date: 2011-05-19