2011 Fiscal Year Final Research Report
In vitro system for spermatogenesis from spermatogonial stem cells
| Project/Area Number |
21592080
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YUMURA Yasushi 横浜市立大学, 市民総合医療センター, 講師 (30522023)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 精子形成 / 精子幹細胞 / 培養 |
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| Research Abstract |
In vitro reconstitution of spermatogenesis is a very difficult task and remained unsuccessful for a long time. We challenged that task using classical organ culture method, called air-liquid interphase. Through testing variety of culture conditions, we found that a serum replacement, KSR, is effective and induced complete spermatogenesis of mouse. Offspring were obtained with the sperm produced in vitro. We also showed that cryopreservation of testis tissue fragment is possible to preserve spermatogenic ability of the tissues.
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