2011 Fiscal Year Final Research Report
Intraoral pH measurement of different carious lesions with qPCR of cariogenic bacteria to differentiate caries activity
| Project/Area Number |
21592413
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KITASAKO Yuichi 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (30361702)
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| Co-Investigator(Kenkyū-buntansha) |
TAGAMI Jyunji 東京医科歯科大学, 医歯学総合研究科, 教授 (50171567)
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| Co-Investigator(Renkei-kenkyūsha) |
MATIN Khairul 東京医科歯科大学, 医歯学総合研究科, 特任講師 (00372433)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 象牙質う蝕 / う蝕活動性 / う蝕部位 / う蝕診断 / pH / う蝕原因細菌 / 遺伝子分析 |
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| Research Abstract |
This study was aimed to measure pH of 103 dentin carious lesions intraorally using a micro-pH sensor, and assess predominant acid-producing cariogenic bacteria in each lesion by real-time PCR to differentiate caries activities. 50 samples were diagnosed as active and 53 as arrested lesions. Statistically significant difference was observed on average surface pH value of approximal and cervical lesions between active and arrested lesions. Prevalence of Lactobacillus spp. was higher in active approximal and cervical lesions than in occlusal and root lesions.
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