Endodontic therapeutic application of electro-magnetic wave irradiation for refractory periapical periodontitis and periapical biofilm

2011 Fiscal Year Final Research Report

• Project/Area Number: 21592423
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: YUMOTO Hiromichi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (60284303)
• Co-Investigator(Kenkyū-buntansha): MATSUO Takashi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (30173800) ; OZAKI Kazumi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (90214121) ; NAKANISHI Tadashi 徳島大学, 病院, 講師 (00217770) ; NAKAE Hideaki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (30227730)
• Project Period (FY): 2009 – 2011
• Keywords: 歯内療法学 / 根尖性歯周炎 / 電磁・高周波 / 細菌 / 骨芽細胞 / 遺伝子発現 / 成長因子 / シグナル伝達

Research Abstract

The Electro-Magnetic Wave Irradiation(EMWI) exhibited great bactericidal activity(> 95. 65%) to kill various oral pathgens. This bactericidal activity by EMWI at 500 kHz(1 sec/ time) increased in an irradiation number-dependent manner. Moreover, EMWI activated MC3T3-E1 cell proliferation. After EMWI(500kHz, 5sec), various growth factors were upregulated. This study suggests that EMWI may contribute to the beneficial effects on bone tissue repair as regenerative therapy and be applicable to refractory periapical periodontitis.

Research Products

• [Journal Article] Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation (2012)
  • Author(s): Hiromichi Yumoto, Toshihiko Tominaga, Kouji Hirao, Tomoko Kimura, Kanako Takahashi, Takashi Sumitomo, Naoki Bando, Takashi Matsuo
  • Journal Title: Journal of Applied Microbiology Volume: (In press)
  • DOI: DOI:10.1111/j.1365-2672.2012.05307.x
• [Journal Article] 電磁波照射の歯内療法への応用. EMAT(Electro-Magnetic Apical Treatment)- (2011)
  • Author(s): 坂東直樹、富永敏彦、湯本浩通、住友孝史、平尾早希、平尾功治、松尾敬志
  • Journal Title: 歯内療法学会雑誌 Volume: Vol.32、No.3 Pages: 184-200
  • Peer Reviewed
• [Presentation] Basic Research aimed at Application of Electro-Magnetic Wave Irradiation for Endodontic Treatment (2011)
  • Author(s): Hiromichi Yumoto
  • Organizer: The 13^<th> Joint-Scientific Meeting of Korean Academy of Conservative Dentistry & Japanese Society of Conservative Dentistry
  • Place of Presentation: Kim Koo Museum & Library(Korea, Seoul)
  • Year and Date: 2011-11-11
• [Presentation] 高周波・電磁波照射による骨芽細胞のGrowth Factorの発現・産生誘導 (2011)
  • Author(s): 湯本浩通
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪国際交流センター(大阪市)
  • Year and Date: 2011-10-20
• [Presentation] 高周波・電磁波照射を歯内療法に応用した症例 (2011)
  • Author(s): 湯本浩通
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート(浦安市)
  • Year and Date: 2011-06-09
• [Presentation] Inactivation and Bactericidal Activity against Oral Bacteria of Electro-Magnetic Wave (2010)
  • Author(s): Hiromichi Yumoto
  • Organizer: 88^<th> General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Centre Convencions Internacional Barcelona(Spain, Barcelona)
  • Year and Date: 2010-07-15
• [Presentation] The effects of Electro-Magnetic-Wave on Oral Bacterial Viability (2010)
  • Author(s): 湯本浩通
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-03-29
• [Presentation] 口腔病原菌に対する高周波・電磁波照射の殺菌効果 (2009)
  • Author(s): 湯本浩通
  • Organizer: 第131回日本歯科保存学会秋季学術大会
  • Place of Presentation: 仙台国際センター(仙台市)
  • Year and Date: 2009-10-29
• [Remarks] 口腔病原菌の殺菌に高周波・電磁波照射が有用、Medical Tribune、2010. 5. 6.
• [Remarks] Tissue Regeneration, NHK World News, 2009. 8. 4