Human arterial chromosomes for new hemophilia therapy

2011 Fiscal Year Final Research Report

• Project/Area Number: 21659238
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Sojo University (2011); Tottori University (2009-2010)
• Principal Investigator: TAKETA Hiroyuki 崇城大学, 生物生命学部, 教授 (60222105)
• Co-Investigator(Renkei-kenkyūsha): KUROSAKI Hajime 鳥取大学, 大学院・医学系研究科, 助教 (70464295)
• Project Period (FY): 2009 – 2011
• Keywords: 分子血栓止血学 / 遺伝子治療 / 組換えタンパク / 血友病 / 遺伝子工学 / 細胞工学

Research Abstract

Human artificial chromosome(HAC) vectors show considerable promise for gene therapy applications as they have the capacity to deliver an extremely large genetic region and are stably maintained independent of the host chromosome, thus diminishing risks of insertional mutagenesis. In this study, we have developed HAC vectors for gene therapy of hemophilia.

Research Products

• [Journal Article] Snake venom metalloproteinases : structure, function and relevance to the mammalian ADAM/ADAMTS family proteins (2012)
  • Author(s): Takeda S, Takeya H, Iwanaga S
  • Journal Title: Biochim Biophys Acta Volume: 1824 Pages: 164-76
  • DOI: doi:10.1016/j.bbapap.2011.04.009.
  • Peer Reviewed
• [Journal Article] Sphingomyelin synthase 1-generated sphingomyelin plays an important role in transferrin trafficking and cell proliferation (2011)
  • Author(s): Shakor AB, Taniguchi M, Kitatani K, Hashimoto M, Asano S, Hayashi A, Nomura K, Bielawski J, Bielawska A, Watanabe K, Kobayashi T, Igarashi Y, Umehara H, Takeya H, Okazaki T.
  • Journal Title: J Biol Chem Volume: 286 Pages: 36053-62
  • Peer Reviewed
• [Journal Article] Integration-free and stable expression of FVIII using a human artificial chromosome (2011)
  • Author(s): Kurosaki H, Hiratsuka M, Imaoka N, Iida Y, Uno N, Kazuki Y, Ishihara C, Yakura Y, Mimuro J, Sakata Y, Takeya H, Oshimura M
  • Journal Title: J Hum Genet Volume: 56 Pages: 727-33
  • DOI: doi:10.1038/jhg.2011.88.
  • Peer Reviewed
• [Journal Article] Effect of dibutyryl cyclic adenosine monophosphate on the gene expression of plasminogen activator inhibitor-1 and tissue factor in adipocytes (2011)
  • Author(s): Taniguchi M, Ono N, Hayashi A, Yakura Y, Takeya H.
  • Journal Title: Thromb Res Volume: 128 Pages: 375-80
  • Peer Reviewed
• [Presentation] 人工染色体ベクターを用いた新規血友病A遺伝子治療法の開発 (2012)
  • Author(s): 矢倉裕奈、黒崎創、石原千恵、香月康宏、土井健史、松下正、武谷浩之、押村光雄
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-14
• [Presentation] ヒト人工染色体を用いた血小板特異的FVIII発現iPS細胞の作製-新規治療法確立へ向けて (2012)
  • Author(s): 矢倉裕奈、黒崎創、石原千恵、香月康宏、土井健史、武谷浩之、押村光雄
  • Organizer: 第34回日本血栓止血学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2012-06-09
• [Presentation] ヒト人工染色体とiPS細胞を用いた新規血友病遺伝子治療法 (2011)
  • Author(s): 矢倉裕奈、石原千恵、藤井昂洸、香月康宏、黒崎創、土井健史、小松則夫、押村光雄、武谷浩之
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] Transcription factor NFAT but not NF-KB is essential for thrombin-induced tissue factor expression (2011)
  • Author(s): Yakura Y, Taniguchi M, Morimoto K, Hayashi A, Takeya H
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: 京都
  • Year and Date: 2011-07-28
• [Presentation] Suppressive actions of fucoidan on lipid droplet formation and plasminogen activator inhibitor-1 expression in adipocytes (2011)
  • Author(s): Hayashi A, Yakura Y, Taniguchi M, Tomita A, Takeya H
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: 京都
  • Year and Date: 2011-07-26
• [Presentation] Regulation of leukotriene production and plasimonen activator inhibitor-1 expression in adipose tissue (2011)
  • Author(s): Hayashi A, Taniguchi M, Yakura Y, Miyabara J, Yamamoto H, Kozutsumi Y, Okazaki T, Takeya H
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: 京都
  • Year and Date: 2011-07-26
• [Presentation] Thrombocytopenia in mice lacking sphingomyelin synthase 1 (2011)
  • Author(s): Fujii, T, Hayashi A, Yakura Y, Taniguchi M, Watanabe K, Kitatani K, Okazaki T, Takeya H
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: 京都
  • Year and Date: 2011-07-25
• [Presentation] Development of human artificial chromosome vectors for gene therapy of hemophilia A (2009)
  • Author(s): Yakura Y, Ishihara C, Hayashi A, Matsui T, Matsumoto S, Kurosaki H, Oshimura M, Doi T, Komatsu N, and Takeya H
  • Organizer: XXIIst Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston
  • Year and Date: 2009-07-16
• [Presentation] ヒト人工染色体を用いた血小板特異的な第VIII因子発現系の確立 (2009)
  • Author(s): 矢倉裕奈、石原千恵、林輝、松井俊樹、松本幸子、黒崎創、押村光雄、土井健史、小松則夫、武谷浩之
  • Organizer: 第32回日本血栓止血学会学術集会
  • Place of Presentation: 北九州
  • Year and Date: 2009-06-04