2011 Fiscal Year Final Research Report
Development of high-speed atomic force/fluorescence microscopy for simultaneous observation of protein dynamics and chemical reactions
| Project/Area Number |
21681017
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 1分子イメージング / ナノ計測 / ナノプローブ / 蛍光顕微鏡 |
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| Research Abstract |
We have developed a combined high-speed atomic force microscopy(AFM) with fluorescence microscopy towards simultaneous observation of single-molecule conformational change of protein and chemical reaction such as ATP binding process. For a stand-alone-type high-speed AFM which can be mounted on an optical microscope, miniaturization of AFM head was carried out and the optical tracking system was developed. Using this system, we succeeded in observing GroEL at a frame rate of 3 frames/s. Further, we confirmed that it enables us to simultaneously obtain AFM and fluorescent images at same area. We also developed a wide-range scanner for fast imaging of bacterial cells and applied it to observation of bacteriolysis reaction of Bacillus subtilis induced by lysozyme.
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