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2010 Fiscal Year Final Research Report

Analysis of neural tissue development using ES cells culture system

Research Project

  • PDF
Project/Area Number 21700367
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Neuroscience in general
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

EIRAKU Mototsugu  The Institute of Physical and Chemical Research, 立体組織形成・解析ユニット, 副ユニットリーダー (40415097)

Project Period (FY) 2009 – 2010
KeywordsES細胞 / 形態形成 / 器官発生 / 試験管内再構築
Research Abstract

In this study, we report the dynamic, autonomous formation of the optic cup (retinal primordium) structure from a three-dimensional culture of mouse ES cell aggregates. ES cell-derived retinal epithelium spontaneously formed hemispherical epithelial vesicles that become patterned along its proximal-distal axis. While the rigid proximal portion differentiated into pigment epithelium, the flexible distal portion progressively folded inward to form a shape reminiscent of the embryonic optic cup, exhibited interkinetic nuclear migration and generated stratified neural retinal tissue, just as seen in vivo. We demonstrate that optic-cup morphogenesis in this simple cell culture depends on an intrinsic self-organizing program involving stepwise and domain-specific regulations in local epithelial properties.

  • Research Products

    (5 results)

All 2011 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Self-organizing optic-cup morphogenesis in three-dimensional culture.2011

    • Author(s)
      Eiraku M, Takata N, Ishibashi H, Kawada M, Sakakura E, Okuda S, Sekiguchi K, Adachi T, Sasai Y
    • Journal Title

      Nature 472

      Pages: 51-56

    • Peer Reviewed
  • [Journal Article] Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals.2011

    • Author(s)
      Danjo T, Eiraku M, Muguruma K, Watanabe K, Kawada M, Yanagawa Y, Rubenstein JL, Sasai Y
    • Journal Title

      J Neurosci. 31

      Pages: 1919-1933

    • Peer Reviewed
  • [Journal Article] Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells.2010

    • Author(s)
      Ohgushi M, Matsumura M, Eiraku M, Murakami K, Aramaki T, Nishiyama A, Muguruma K, Nakano T, Suga H, Ueno M, Ishizaki T, Suemori H, Narumiya S, Niwa H, Sasai Y
    • Journal Title

      Cell Stem Cell 7

      Pages: 225-239

    • Peer Reviewed
  • [Presentation] 神経前駆細胞の細胞周期進行が脳形成に持つ意義の解明に向けて2009

    • Author(s)
      永楽元次、笹井芳樹
    • Organizer
      第32回日本神経科学大会シンポジウム,演題「ES細胞からの大脳皮質神経組織分化について」
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-09-17
  • [Remarks] ホームページ等

    • URL

      http://www.riken.go.jp/r-world/research/lab/cdb/fdta/index.html

URL: 

Published: 2012-02-13   Modified: 2016-04-21  

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