2010 Fiscal Year Final Research Report
Protein patterning and parallel analysis of DNA molecules by integrating nanoimprint lithography (NIL) and UV lithography (UVL)
| Project/Area Number |
21710129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Kyoto University |
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Principal Investigator |
YOKOKAWA Ryuji Kyoto University, 大学院・工学研究科・マイクロエンジニアリング専攻, 助教 (10411216)
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| Co-Investigator(Renkei-kenkyūsha) |
笠原 賢洋 立命館大学, 生命科学部, 准教授 (70361748)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ナノインプリント / MicroTAS / MEMS / 分子操作 / DNA |
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| Research Abstract |
We modified two ends ofλDNA by digoxigenin (DIG) and biotin for single molecule manipulation. One end labeled with DIG was immobilized on the glass surface coated by anti-DIG antibody, and the other end labeled with biotin was manipulated by gliding microtubules modified with streptavidin. After stretchingλDNA, a restriction enzyme was applied to visualize cleaving of the DNA molecule at single molecule level. To achieve highly parallel and massive DNA manipulation, we fabricated micro/nano scale perfusable multi-scale channel structures. Integration of nanoimprint lithography (NIL) and UV lithography (UVL) realized such a complex and multi-scale structures. It was evaluated by implementing a pressure-driven flow system and protein assay.
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