2010 Fiscal Year Final Research Report

Building of a cassava information resource and understanding of environmental stress responses

Research Project

Project/Area Number	21710205
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Applied genomics
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	SAKURAI Tetsuya The Institute of Physical and Chemical Research, ゲノム情報統合化ユニット, ユニットリーダー (90415167)
Project Period (FY)	2009 – 2010
Keywords	植物 / 作物 / ゲノム / トランスクリプトーム / 環境応答 / データベース
Research Abstract	We developed a oligo custom microarray chip which includes approximately 22,000 60-mer probes. The microarray chip was designed by using the ESTs from GenBank (NCBI) and flanking sequences of RIKEN cassava full-length cDNA. Using the microarray chip, we were able to outline gene expression among 3 cassava accession and the 2 stress experimental conditions. Moreover, we performed gene expression profiling via CAGE method, obtained the different gene expression profiles from the 2 methodological aspects.

(3 results)