Chemical genetic research on cellular membrane using theonellamide.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21710227
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Kyoto University
• Principal Investigator: NISHIMURA Shinichi Kyoto University, 薬学研究科, 助教 (30415260)
• Project Period (FY): 2009 – 2010
• Keywords: 海洋天然物 / 脂質生物学 / 分裂酵母 / ケミカルジェネティクス / ケミカルゲノミクス

Research Abstract

Theonellamides are known to recognize membrane sterol molecules and its consequences are unique cellular morphological changes. However, molecular mechanisms underlying the phenomena have not been clarified. In this study, we tried to uncover the mechanisms by genetic and chemical genetic approaches. We identified several genes/proteins whose mutation affected the sensitivity of cells to theonellamide, while some of them were revealed to be involved in an unknown system to orchestrate the events on/around the plasma membrane.

Research Products

• [Journal Article] 細胞膜を撹乱する海洋天然物-セオネラミドはステロールに結合し、異常な細胞壁合成を誘導する- (2011)
  • Author(s): 西村慎一, 掛谷秀昭, 吉田稔
  • Journal Title: 化学と生物 49 Pages: 295-297
• [Journal Article] プロファイリングによる化合物の作用解析 (2011)
  • Author(s): 西村慎一, 吉田稔
  • Journal Title: 化学 66 Pages: 51-56
• [Journal Article] 新規有用天然有機化合物の開拓とケミカルバイオロジー研究 (2010)
  • Author(s): 掛谷秀昭, 西村慎一
  • Journal Title: 有機合成化学協会誌 68 Pages: 490-500
  • Peer Reviewed
• [Journal Article] Marine antifungal theonellamides target 3β-hydroxysterol toactivate Rho1 signaling. (2010)
  • Author(s): Nishimura, S., et al.
  • Journal Title: Nat.Chem.Biol. 6 Pages: 519-526
  • Peer Reviewed
• [Presentation] Modes of action oftheonellamides, antifungal bicyclicpeptides from a marine sponge. (2010)
  • Author(s): Nishimura, S., Matsunaga, S., Kakeya,H., Yoshida, M.
  • Organizer: Pacifichem2010
  • Place of Presentation: Hawaii,U.S.A.
  • Year and Date: 20101215-20101220
• [Presentation] ステロールを標的とするtheonellamideの作用機序解析 (2010)
  • Author(s): 西村慎一, 越智順子, 有田裕子, 松山晃久, 松永茂樹, 吉田稔, 掛谷秀昭
  • Organizer: 第5回日本ケミカルバイオロジー学会年会
  • Place of Presentation: 神奈川
  • Year and Date: 20100518-20100519
• [Presentation] 海産抗真菌物質theonellamideの作用機序解明 (2010)
  • Author(s): 西村慎一, 越智順子, 有田裕子, 松山晃久, 吉田稔, 掛谷秀昭.
  • Organizer: 日本薬学会近畿支部会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-30
• [Presentation] Regulation of sterol localization revealed by chemical genetics using the onellamide. (2009)
  • Author(s): Nishimura, S., Matsuyama, A., Yoshida,M., Matsunaga, S., Kakeya, H.
  • Organizer: The 5th International Fission Yeast Meeting.
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 20091026-20091031
• [Presentation] ステロールを標的とするtheonellamide類が誘導する分裂酵母の形態異常 (2009)
  • Author(s): 西村慎一, 松山晃久, 松永茂樹, 掛谷秀昭, 吉田稔
  • Organizer: 第42回酵母遺伝学フォーラム研究報告会
  • Place of Presentation: 茨城
  • Year and Date: 20090728-20090730