2010 Fiscal Year Final Research Report
Structure-function analysis of budding yeast cohesin complex involved in sister chromatid cohesion
| Project/Area Number |
21770172
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
NISHINO Tatsuya National Institute of Genetics, 分子遺伝研究部門, 助教 (50533155)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 構造生物学 / X線構造解析 / 高速AFM姉妹染色体連結 |
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| Research Abstract |
Cohesin complex is involved in holding replicated sister chromatids. Sister chromatid cohesion is conserved among eukaryotes and is required for faithful segregation of chromosomes. Cohesin complex is comprised of Smc proteins and non-Smc proteins. However, the structure-function relationship of this complex remains elusive. We have used high speed atomic force microscopy to visualize the cohesin complex. We could clearly see ATPase domain, coiled coil region and dimerization domain. We found that this domain organization is conserved among other Smc complexes and the stability of coiled coil region was observed.
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