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2010 Fiscal Year Final Research Report

Analysis of the relationship between herbicide resistance mechanism and biophoton generation

Research Project

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Project/Area Number 21780018
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Crop science/Weed science
Research InstitutionShizuoka Prefectural Research Institute of Agriculture and Forestry

Principal Investigator

NUKUI Hideki  Shizuoka Prefectural Research Institute of Agriculture and Forestry, 花き科, 主任研究員 (10426463)

Project Period (FY) 2009 – 2010
Keywords除草剤抵抗性 / バイオフォトン
Research Abstract

The generating mechanism of herbicide responsive photon emission from cultured rice cells was analyzed. 50-100 ppm bensulfronmethyl (BSM) treatment induced an increment of photon emission from rice cells. Emission patterns of 18 tested herbicides were different from each other. CYP81A6, a cytochrome P450 gene reported to be responsible for BSM tolerance in rice, was isolated from the rice cells, and was silenced by RNAi. Responses of CYP81A6-silenced cell line to BSM and branched chain amino acids revealed that the BSM detoxifying reaction by cytochrome P450 is involved in BSM responsive photon emission from rice cells.

  • Research Products

    (3 results)

All 2011 2010 Other

All Presentation (2 results) Remarks (1 results)

  • [Presentation] Involvement of cytochrome P450 in bensulfronmethyl responsive photon emission from rice cells2011

    • Author(s)
      Nukui, H., Iyozumi, H., Kato, K., Kageyama, C.
    • Organizer
      51^<st> Annual Meeting of the Weed Science Society of America
    • Place of Presentation
      Hilton Portland & Executive Tower, Portland, Oregon
    • Year and Date
      20110000
  • [Presentation] イネ培養細胞の除草剤応答発光2010

    • Author(s)
      貫井秀樹、伊代住浩幸、加藤公彦
    • Organizer
      日本雑草学会
    • Place of Presentation
      AOSSA福井市地域交流プラザ・福井県県民ホール
    • Year and Date
      20100000
  • [Remarks] ホームページ等

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Published: 2012-02-13   Modified: 2016-04-21  

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