2010 Fiscal Year Final Research Report
Analysis of the relationship between herbicide resistance mechanism and biophoton generation
| Project/Area Number |
21780018
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Crop science/Weed science
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| Research Institution | Shizuoka Prefectural Research Institute of Agriculture and Forestry |
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Principal Investigator |
NUKUI Hideki Shizuoka Prefectural Research Institute of Agriculture and Forestry, 花き科, 主任研究員 (10426463)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 除草剤抵抗性 / バイオフォトン |
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| Research Abstract |
The generating mechanism of herbicide responsive photon emission from cultured rice cells was analyzed. 50-100 ppm bensulfronmethyl (BSM) treatment induced an increment of photon emission from rice cells. Emission patterns of 18 tested herbicides were different from each other. CYP81A6, a cytochrome P450 gene reported to be responsible for BSM tolerance in rice, was isolated from the rice cells, and was silenced by RNAi. Responses of CYP81A6-silenced cell line to BSM and branched chain amino acids revealed that the BSM detoxifying reaction by cytochrome P450 is involved in BSM responsive photon emission from rice cells.
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