2010 Fiscal Year Final Research Report

Analysis of the relationship between herbicide resistance mechanism and biophoton generation

Research Project

Project/Area Number	21780018
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Crop science/Weed science
Research Institution	Shizuoka Prefectural Research Institute of Agriculture and Forestry
Principal Investigator	NUKUI Hideki Shizuoka Prefectural Research Institute of Agriculture and Forestry, 花き科, 主任研究員 (10426463)
Project Period (FY)	2009 – 2010
Keywords	除草剤抵抗性 / バイオフォトン
Research Abstract	The generating mechanism of herbicide responsive photon emission from cultured rice cells was analyzed. 50-100 ppm bensulfronmethyl (BSM) treatment induced an increment of photon emission from rice cells. Emission patterns of 18 tested herbicides were different from each other. CYP81A6, a cytochrome P450 gene reported to be responsible for BSM tolerance in rice, was isolated from the rice cells, and was silenced by RNAi. Responses of CYP81A6-silenced cell line to BSM and branched chain amino acids revealed that the BSM detoxifying reaction by cytochrome P450 is involved in BSM responsive photon emission from rice cells.

(3 results)