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2010 Fiscal Year Final Research Report

Functional and structural analyses of the archaeal L-proline dehydrogenase complexes

Research Project

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Project/Area Number 21780099
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionSaga University

Principal Investigator

KAWAKAMI Ryushi  Saga University -> 佐賀大学, 総合分析実験センター, 助教 (90380120)

Project Period (FY) 2009 – 2010
Keywords酵素化学 / 構造生物学
Research Abstract

Two different types of ProDHs (α_4β_4 and αβγδ type) were distributed in the hyperthermophilic archaea. Functional and structural characteristics of both ProDHs were studied. Structure determination at high resolution of α_4β_4-type ProDH which the ICS was not broken, and mutational analysis of intersubunit disulfide bond in α subunit of α_4β_4-type ProDH were performed. Screening of crystallization condition of αβγδ type ProDH was also performed by preparing the ISC mutant enzymes. And the enzymatic properties of β subunits of both types of ProDHs were analyzed.

Research Products

(5 results)

All 2011 2010 2009

All Presentation

  • [Presentation] 超好熱アーキアのL-プロリン脱水素酵素サブユニットの機能解析2011

    • Author(s)
      川上竜巳、野口千明、東麻梨江、櫻庭春彦、大島敏久
    • Place of Presentation
      京都女子大学・京都(発表会は中止)
    • Year and Date
      2011-03-26
  • [Presentation] 超好熱アーキアのL-プロリン脱水素酵素サブユニットの比較解析2010

    • Author(s)
      野口千明、東麻梨江、櫻庭春彦、大島敏久、川上竜巳
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド・兵庫
    • Year and Date
      2010-12-10
  • [Presentation] 超好熱アーキアPyrococcus horikoshiiのα_4β_4型色素依存性L-プロリン脱水素酵素複合体のシステイン残基の役割2010

    • Author(s)
      川上竜巳、櫻庭春彦、大島敏久
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド・兵庫
    • Year and Date
      2010-12-10
  • [Presentation] 超好熱アーキアのL-プロリン脱水素酵素サブユニットの機能解析2010

    • Author(s)
      野口千明、東麻梨江、櫻庭春彦、大島敏久、川上竜巳
    • Organizer
      日本農芸化学会西日本支部大会
    • Place of Presentation
      崇城大学・熊本
    • Year and Date
      2010-09-18
  • [Presentation] 超好熱アーキア由来色素依存性L-プロリン脱水素酵素複合体のL-プロリン脱水素酵素サブユニットの機能解析2009

    • Author(s)
      東麻梨江、川上竜巳
    • Organizer
      蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      唐津シーサイドホテル・佐賀
    • Year and Date
      2009-09-10

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Published: 2012-02-12   Modified: 2016-04-21  

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