2010 Fiscal Year Final Research Report
Triggering mechanism on the symplectin bioluminescence by reactive oxygen species
| Project/Area Number |
21780111
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
KUSE Masaki Nagoya University, 物質科学国際研究センター, 助教 (40335013)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 生物発光 / 発光タンパク質 / 活性酸素 / 発光速度 / クロモフォア |
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| Research Abstract |
Triggering mechanism of photoproteins has been investigated. The photoproteins have chromophore which is constituted from dehydrocoelenterazine (DCL). It demonstrated that oxo-complex of Fe ion made the luminescence speed fast, on the other hand, peroxide of hypochlorite made the luminescence speed slow. This specific reaction rate was proved to be useful for the identification of reactive oxygen species by using photoproteins. A convenient synthetic method was also established to prepare many kinds of DCLs.
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