Elucidation of AHL mimics secreted from plant roots

2010 Fiscal Year Final Research Report

• Project/Area Number: 21780114
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: KAI Kenji Osaka Prefecture University, 生命環境科学研究科, 助教 (40508404)
• Project Period (FY): 2009 – 2010
• Keywords: アシルホモセリンラクトンミミック / アルファルファ / グラム陰性細菌 / クオラムセンシング

Research Abstract

I optimized the method to obtain active compound that mimics bacterial quorum sensing signal from root exudates of alfalfa plant. Activated charcoal effectively recovered the compounds from hydroponic culture of alfalfa. Finally, the compound could be purified by several chromatographic separation, and the structure was elucidated based on the spectroscopic and chemical methods.