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2010 Fiscal Year Final Research Report

The molecular mechanism of catch contraction based on the structural characteristics of twitchin of bivalve smooth muscle

Research Project

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Project/Area Number 21780195
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionMie University

Principal Investigator

FUNABARA Daisuke  Mie University, 大学院・生物資源学研究科, 准教授 (00335150)

Project Period (FY) 2009 – 2010
Keywordsキャッチ収縮 / 二枚貝 / ミオシン / アクチン / トゥイッチン / リン酸化
Research Abstract

Myosin S1 of catch muscle of mussel Mytilus galloprovincialis was expressed using a protein expression system using the wheat germ extract. The myosin binding regions on the twitchin molecule were predicted with the ITC analysis using the peptides designed according to the sequence of twitchin and the myosin loop 2 peptide. The expression of myosin and paramyosin genes in catch muscles of mussel was suppressed by the RNA interference methods.

Research Products

(5 results)

All 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] 二枚貝キャッチ運動の分子メカニズム2009

    • Author(s)
      舩原大輔
    • Journal Title

      溶接学会誌 78

      Pages: 25-29

  • [Journal Article] Myosin loop 2 is involved in the formation of a trimeric complex of twitchin, actin and myosin.2009

    • Author(s)
      Daisuke Funabara, Rika Osawa, Miki Ueda, Satoshi Kanoh, David J.Hartshorne,Shugo Watabe.
    • Journal Title

      J.Biol.Chem. 284

      Pages: 18015-18020

    • Peer Reviewed
  • [Presentation] RNA干渉法によるキャッチ筋タンパク質ノックダウン二枚貝作出の検討2010

    • Author(s)
      長尾知樹・六分一早希・舩原大輔・加納哲
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2010-09-24
  • [Presentation] キャッチ筋トゥイッチンの結合によるF-アクチンの構造変化2010

    • Author(s)
      横谷沙季・舩原大輔・渡部終五・加納哲
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2010-03-27
  • [Presentation]

    • Author(s)
      .

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Published: 2012-02-12   Modified: 2016-04-21  

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