2010 Fiscal Year Final Research Report

Signaling pathway in amino acids mediated suppression of muscle proteolysis

Research Project

Project/Area Number	21780256
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Applied animal science
Research Institution	National Agricultural Research Organization
Principal Investigator	NAKASHIMA Kazuki 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・分子栄養研究チーム, 主任研究員 (70370583)
Project Period (FY)	2009 – 2010
Keywords	アミノ酸 / タンパク質分解 / タンパク質分解関連遺伝子発現 / 筋肉細胞培養
Research Abstract	The present study addressed the role of amino acids as a regulator of proteolysis in myotubes. Leucine and glutamine suppressed proteolysis, while also decreasing expression of atrogin-1 mRNA through the L- system amino acid transporter (LAT1). Amino acids did not activate AKT-FOXO signaling pathway, but inhibiting proteolysis. Thus, an important component of muscle proteolysis inhibition by leucine or amino acids, through LAT1 but not AKT-FOXO signaling pathway, is its ability to suppress muscle protein degradation.