Establishment of substrates exploration of protozoan enzymes associated with invasion into host cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 21780263
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: The University of Tokyo
• Principal Investigator: KATO Kentaro The University of Tokyo, 大学院・農学生命科学研究科, 助教 (30401178)
• Project Period (FY): 2009 – 2010
• Keywords: 原虫 / 基質 / 酵素 / 宿主細胞侵入

Research Abstract

We have got some results that protozoa enzymes including protein kinases take parts in the important signal transductions for parasites to transfer from a stage to the next stage by analyzing the substrates and functions of enzymes relating with calcium signaling in their complicated life cycles. These results lead to the new anti-protozoan drug developments to block the protozoa-specific life cycle by inhibiting the functions of the important protozoan enzymes in the stage transfer.

Research Products

• [Journal Article] Identification of Toxonlasma cAMP dependent protein kinase and its role in the tachyzoite growth. (2011)
  • Author(s): Kurokawa H, Kato K (corresponding author), Iwanaga T, Sugi T, Sudo A, Kobayashi K, Gong H, Takemae H, Recuenco FC, Horimoto T, Akashi H.
  • Journal Title: PLoS One. In press.
  • Peer Reviewed
• [Journal Article] 1NM-PP1 treatment of mice infected with Toxonlasma (2011)
  • Author(s): Sugi T, Kato K (corresponding author), Kobayashi K, Kurokawa H, Takemae H, Gong H, Recuenco FC, Iwanaga T, Horimoto T, Akashi H.
  • Journal Title: J Vet Med Sci. In press.
  • Peer Reviewed
• [Journal Article] Bovine viral diarrhea virus nonstructural protein 5A interacts with NIK and IKKβ-binding protein (NIBP). (2010)
  • Author(s): Zahoor MA, Yamane, D, Mohamed YM, Suda Y, Kobayashi K, Kato K, Tohya T, Akashi H.
  • Journal Title: J Gen Virol. 91 Pages: 1939-1948
  • Peer Reviewed
• [Journal Article] Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxonlasma gondii CDPK1 in the invasion step. (2010)
  • Author(s): Sugi T, Kato K (corresponding author), Kobayashi K, Watanabe S, Kurokawa H, Gong H, Pandey K, Takemae H, Akashi H.
  • Journal Title: Eukaryot Cell. 9 Pages: 667-670
  • Peer Reviewed
• [Journal Article] Molecular analyses of Toxonlasma calmodulin-like domain protein kinase isoform 3. (2009)
  • Author(s): Sugi T, Kato K (corresponding author), Kobayashi K, Pandey K, Takemae H, Kurokawa H, Tohya Y, Akashi H.
  • Journal Title: Parasitol Int. 58 Pages: 416-423
  • Peer Reviewed
• [Journal Article] Characterization of plasmodium falciparum calcium-dependent protein kinase 4. (2009)
  • Author(s): Kato K (corresponding author), Sudo A, Kobayashi K, Sugi T, Tohya Y, Akashi H.
  • Journal Title: Parasitol Int. 58 Pages: 394-400
  • Peer Reviewed
• [Presentation] Characterization of Plasmodium falciparum calcium-dependent protein kinase 4 (2010)
  • Author(s): Kentaro Kato, Atsushi Sudo, Kyosuke Kobayashi, Tatsuki Sugi, Yukinobu Tohya, Hiroomi Akashi.
  • Organizer: XIIth International Congress of Parasitology (ICOPAXII)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20100800
• [Presentation] トキソプラズマ原虫におけるcAMP依存性プロテインキナーゼの機能解析 (2010)
  • Author(s): 黒川瞳、加藤健太郎、首藤篤史、小林郷介、杉達紀、竹前等、ゴン海燕、堀本泰介、明石博臣
  • Organizer: 第18回分子寄生虫ワークショップ
  • Place of Presentation: 群馬
  • Year and Date: 20100800
• [Presentation] ASKA法によって明らかにされた、トキソプラズマ原虫CDPK1による滑走運動制御 (2010)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、明石博臣
  • Organizer: 第79回日本寄生虫学会
  • Place of Presentation: 北海道
  • Year and Date: 20100500
• [Presentation] マラリア原虫独自酵素によるその巧妙なライフサイクルコントロール (2010)
  • Author(s): 加藤健太郎
  • Organizer: 第149回日本獣医学会,第1回獣医寄生虫学奨励賞受賞
  • Place of Presentation: 東京
  • Year and Date: 20100300
• [Presentation] トキソプラズマ原虫CDPKの原虫生活環における役割 (2009)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、遠矢幸伸、明石博臣
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
  • Year and Date: 20090900
• [Presentation] マラリア原虫のPfCDPK4の有性生殖期における発現解析 (2009)
  • Author(s): 加藤健太郎、小林郷介、杉達紀、遠矢幸伸、明石博臣
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
  • Year and Date: 20090900
• [Presentation] アピコンプレクサ門原虫プロテインキナーゼ特異的阻害剤探索 (2009)
  • Author(s): 杉達紀、加藤健太郎、小林郷介、黒川瞳、明石博臣
  • Organizer: 第17回分子寄生虫ワークショップ
  • Place of Presentation: 群馬
  • Year and Date: 20090800
• [Presentation] 牛ウイルス性下痢ウイルスによるスフィンゴ脂質代謝制御を介したウイルス複製機構の解析 (2009)
  • Author(s): 山根大典、加藤健太郎、遠矢幸伸、明石博臣
  • Organizer: 第147回日本獣医学会
  • Place of Presentation: 栃木
  • Year and Date: 20090400
• [Patent(Industrial Property Rights)] Toxoplasma gondii RON4-binding molecules by retrovirus-mediated expression cloning (2010)
  • Inventor(s): Kentaro Kato, Taisuke Horimoto, Hiroomi Akashi, Hitoshi Takemae
  • Industrial Property Rights Holder: Kentaro Kato, Taisuke Horimoto, Hiroomi Akashi, Hitoshi Takemae
  • Industrial Property Number: 特許権,US61/447330
  • Filing Date: 2010-02-28