2010 Fiscal Year Final Research Report
Inhibition of quorum sensing by recombinant R protein.
| Project/Area Number |
21780264
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MINEMATSU Takeo The University of Tokyo, 大学院・医学系研究科, 特任助教 (00398752)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 病原微生物 |
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| Research Abstract |
In order to develop the novel technique to prevent the bacterial infection, I tried to synthesize the recombinant protein of receptor for quorum sensing-signaling molecule (AHL), LasR. The analysis of amino acids of cloned lasR gene indicated that LasR consists of AHL-binding domain and DNA-binding domain. The recombinant LasR was successfully synthesized by in vitro system using insect cell extraction, while LasR was rapidly digested when the protein was synthesized in E. coli competent cells or cultured cells. In addition, the effect of AHL to promote the wound healing was discovered during the process of this research.
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