2010 Fiscal Year Final Research Report
Development of a nonhuman primate model for dengue virus infection
| Project/Area Number |
21780274
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
KOBAYASHI Takeshi Kyoto University, ウイルス研究所, 助教 (90324847)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | デングウイルス / サルモデル |
|---|
| Research Abstract |
To develop a nonhuman primate model for dengue virus (DV) infection, rhesus monkeys were inoculated with DV types 1 and 2. The DV types 1 and 2 induced viremia with duration from 2 to 3 days and a mean peak titer of 4×10^3 RNA copies/ml but infected monkeys showed no sign of dengue diseases even after inoculation of high virus doses. DV type 2 effectively replicates in MDM cells derived from bonnet monkeys compared to those derived from rhesus monkeys. These results suggest that bonnet monkey may be a useful model to study dengue disease.
To improve a reverse genetics for tick-borne encephalitis virus (TBEV) which belongs to the same member of the Flavivirus genus as DV, we have constructed a plasmid-based infectious clone encoding full-length TBEV cDNA under control of the minimum cytomegalovirus promoter. Transfection of cells with the plasmid resulted in the production of infectious TBEV at high levels. Furthermore, we have developed homologous recombination-based reverse genetics for TBEV and DV. The reverse genetics approaches described here can be exploited for studies of DV replication and pathogenesis as powerful tools.
|
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Strategies to Develop Recombinant Rotavirus2009
Author(s)
Shane D.Trask, Zenobia F.Taraporewala, Takeshi Kobavashi, Andrew J.Rolle, Terence S.Dermody, John T.Patton.
Organizer
American Society for Virology 28th Annual meeting, Vancouver, BC, Canada
Place of Presentation
poster presentation
Year and Date
20090711-20090715
-
-
[Remarks] ホームページ等
