2010 Fiscal Year Final Research Report
Systems biology approach in unraveling the mechanism of cellular toxicity of auxinic herbicide
Project/Area Number |
21780305
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Applied molecular and cellular biology
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Research Institution | Iwate University |
Principal Investigator |
RAHMAN Abidur Iwate University, 農学部, 准教授 (30447114)
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Project Period (FY) |
2009 – 2010
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Keywords | herbicide / 2,4-D / auxin / actin / apoptosis / transcriptome |
Research Abstract |
The auxinic herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used to kill the weeds in crop fields. However, the molecular mechanism how it kills the dicot remains elusive. Previously, we provided evidence that 2,4-D degrades the cell cytoskeletal machinery and induces apoptosis through a novel auxin independent pathway (Rahman et al., 2007 ; Takahashi et al., manuscript in preparation). These results confirmed that the herbicidal action of 2,4-D is tightly linked to cellular toxicity instead of commonly believed acute auxin toxicity. To further clarify the mode of action of 2,4-D and unravel the molecular mechanism of its cellular toxicity, a systems biology approach was taken, which includes omics, bioinformatics and reverse genetics. Comparative transcriptome analysis between IAA and 2,4-D, identified distinct set of genes that are specifically responsive to 2,4-D. The Gene Ontology annotation analyses of these 2,4-D responsive genes reveal a group of genes that are linked to cell cytoskeletal component and apoptosis, confirming that 2,4-D kills the plant through actin degradation and subsequent apoptosis of cells. To understand the functional roles of the proteins that regulate this pathway, a reverse genetic approach has been taken.
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Research Products
(18 results)
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[Journal Article] Shootward and rootward : peak terminology for plant polarity.2010
Author(s)
Baskin, TI, Peret, B, Balu?ka, F, Benfey, PN, Bennett, M, Forde, BG, Gilroy, S, Helariutta, Y, Hepler, PK, Leyser, O, Masson, PH, Muday, GK, Murphy, AS., Poethig, S, Rahman, A, Roberts, K, Scheres, B, Sharp, RE, Somerville, C.
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Journal Title
Trends in Plant Science 15
Pages: 593-594
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