2010 Fiscal Year Final Research Report

Therapeutic application to neonatal PVL of amnion-derived cells

Research Project

Project/Area Number	21791029
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Embryonic/Neonatal medicine
Research Institution	National Cardiovascular Center Research Institute
Principal Investigator	SASAKI Yoshihito 独立行政法人国立循環器病研究センター, 周産期・婦人科部 (50396389)
Project Period (FY)	2009 – 2010
Keywords	胎児医学
Research Abstract	We have obtained human amnion during caesarian section from 20 volunteers. After the culture, amniotic cells were sorted by flow cytometry. The cell fraction that we consider containing stem cells is no more than 0.02% of total cells. A neonatal rat model with hypoxic-ischemic brain injury was prepared. We injected these cells suspended in a solution into the lateral ventricle of the brain. We also applied the cells as a form of cell sheet directly onto the injured cerebral cortex. Neuroprotective effects of the cell therapies have not been observed so far. We continue analyzing other methods of cell delivery and other cell sources.