The function of newly identified lymphoid cell ectopically expressing keratin 5

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791076
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: AZUKIZAWA Hiroaki Osaka University, 大学院・医学系研究科, 助教 (10379240)
• Project Period (FY): 2009 – 2010
• Keywords: 皮膚自己免疫疾患 / 樹状細胞 / 動物モデル / 抗原提示細胞 / 未熟B細胞

Research Abstract

>To study the role of ectopic epidermal auto-antigen expression, K5-Cre x CAG-CAT-EGFP double transgenic mice that express enhanced green fluorescence protein (GFP) under the control of keratin 5 promoter were employed. Unexpectedly, GFP^<high> cells distinct from migratory DC were found not only in skin-draining LN, but also in mesenteric LN, spleen, peripheral blood and bone marrow, indicating that bone marrow-derived circulating cells express keratin 5. Interestingly, GFP^<high> cells strongly expressed MHC class II and were enriched from selected CD45R (B220) + and IgD- population. GFP^<high> cells crosspresented ovalbumin (OVA) as well as immature B cells and pDC in vitro, whereas GFP^<high> cells and immature B cells, but not pDC, presented OVA to CD4 T cells. These results indicate that immature B cell-like circulating lymphoid cell is an antigen-presenting cell having a unique characteristic to express ectopic self-antigen.

Research Products

• [Journal Article] BerBerich-Siebelt F, Lutz MB. Steady state migratory Rel-B+ Langerin+ dermal dendritic cells mediate peripheral induction of antigen-specific CD4+CD25+ Foxp3+ regulatory T cells. (2011)
  • Author(s): Azukizwa H., Doehler A, Kanazawa N, Nayak A, Lipp M, Malissen B, Autenrieth I, Katayama I, Rieman M, Weih F.
  • Journal Title: Eur J Immunol in press
  • Peer Reviewed
• [Journal Article] 中毒性表皮壊死症(TEN)の発症機序 (2010)
  • Author(s): 小豆澤宏明
  • Journal Title: Journal of Environmental Dermatology and Cutaneous Allergology 4(3) Pages: 137-142
  • Peer Reviewed
• [Journal Article] Antigen-specific CD4 cells assist CD8 T-effector cell ineliminating keratinocytes. (2010)
  • Author(s): Broom JK, Lew AM, Azukizwa H., Kenna TJ, Leggatt zer IH.
  • Journal Title: J Invest Dermatol 130(6) Pages: 1581-9
  • Peer Reviewed
• [Journal Article] Revisiting the tolerogenicity of epidermal Langerhans cells. (2010)
  • Author(s): Lutz MB, Dohler A, Azukizwa H.
  • Journal Title: Immunol Cell Biol 88(4) Pages: 381-6
  • Peer Reviewed
• [Journal Article] Antigen-specific CD8 T cells celiminate antigen-bearing keratinocytes with clonogenic potential via an IFN-gamma-dependent mechanism. (2010)
  • Author(s): De Kluyver RL, Moritz L, Harris CA, Azukizwa H, Frazer IH.
  • Journal Title: J Invest Dermatol 130(7) Pages: 1841-8
  • Peer Reviewed
• [Journal Article] CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells. (2009)
  • Author(s): Henri S, Poulin LF, Tamoutounour S, Ardouin L, Guilliams M, de Bovis B, Devilard E, Viret C, Azukizwa H, Kissenpfennig A, Malissen B.
  • Journal Title: J Exp Med 207(1) Pages: 189-206
  • Peer Reviewed
• [Presentation] Immature B cell-like circulating lymphoid cells express keratin 5 ectopically and present exogenous antigen comparable to plasmacytoid dendritic cell. (2010)
  • Author(s): Hanafusa T, Azukizwa H, Katayama I.
  • Organizer: 35^<th> Annual Meeting of the Japanese Society for Investigative Dermatology.
  • Place of Presentation: Wakayama
  • Year and Date: 20101203-20101205
• [Presentation] Immature B cell-like circulating lymphoid cells express keratin 5 ectopically and present exogenous antigen comparable to plasmacytoid dendritic cell. (2010)
  • Author(s): Hanafusa T, Azukizwa H, Katayama I.
  • Organizer: 11^<th> International Symposium on Dendritic cells in Fundamental and Clinical Immunology.
  • Place of Presentation: Lugano
  • Year and Date: 20100926-20100930
• [Presentation] CD207+ CD103+ DDCs cross-present keratinocyte-derived antigens irrespective of the presence of LCs. (2010)
  • Author(s): Henri S, Tamoutounour S, Poulin LF, Azukizwa H, Kissenpfennig A, Malissen B.
  • Organizer: 11^<th> International Symposium on Dendritic cells in Fundamental and Clinical Immunology.
  • Place of Presentation: Lugano
  • Year and Date: 20100926-20100930
• [Presentation] Immature B cell-like circulating lymphoid cells express keratin 5 ectopically and present exogenous antigen comparable to plasmacytoid dendritic cell. (2010)
  • Author(s): Hanafusa T, Azukizwa H, Katayama I.
  • Organizer: 14^<th> International Congress of Immunology.
  • Place of Presentation: Kobe
  • Year and Date: 20100822-20100827
• [Presentation] 皮膚科研究の最前線皮膚免疫・アレルギーの進歩皮膚樹状細胞の役割について (2010)
  • Author(s): 小豆澤宏明
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20100416-20100418
• [Presentation] Ectopic expression of keratin 5 in lymphoid cell. (2009)
  • Author(s): Hanafusa T, Azukizwa H, Katayama I.
  • Organizer: 日本研究皮膚科学会総会
  • Place of Presentation: 博多
  • Year and Date: 20091204-20091206
• [Presentation] Ectopic expression of keratin 5 in lymphoid cell. (2009)
  • Author(s): Azukizwa H, Hanafusa T, Katayama I.
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20091202-20091204