2010 Fiscal Year Final Research Report
Hydrogen Sulfide Attenuates the Development of Skin Fibrosis in Tight-Skin Mice
Project/Area Number |
21791083
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Dermatology
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Research Institution | Nagasaki University |
Principal Investigator |
MUROI Eiji Nagasaki University, 大学院・医歯薬学総合研究科, 助教 (80420636)
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Project Period (FY) |
2009 – 2010
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Keywords | 全身性強皮症 / 硫化水素 / 酸化ストレス / 自己抗体 / 抗酸化酵素 |
Research Abstract |
Oxidative stress has been suggested to contribute to clinical manifestations associated with systemic sclerosis (SSc). Hydrogen sulfide (H_2S) can neutralize a variety of reactive species and thereby exhibits the cytoprotective effects. In this study, we assessed the therapeutic effect of H_2S on a tight-skin (TSK) mouse, a mouse model for human SSc. NaHS treatment significantly reduced hypodermal thickness by 70%. NaHS treatment decreased anti-ssDNA IgM antibody levels in wild type and TSKmice. NaHS significantly increased HO-1 gene expression in the TSK skin to a similar level of the wild type skin. NaHS treatment significantly reduced eNOS expression in the TSK/+ skin. Furthermore, proa2 (I) collagen gene expression by cultured TSK fibroblasts was inhibited by NaHS treatment. In the current study, H_2S showed remarkable effectiveness in TSK/+ mice, suggesting that oxidative stress is critical for the development of skin fibrosis in TSK/+ mice.
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Research Products
(5 results)