Basic Research in Bioimaging for Expression of Hepatocyte Surface Membrane Protein Using Magnetic Resonance Imaging

2011 Fiscal Year Final Research Report

• Project/Area Number: 21791191
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Osaka University
• Principal Investigator: ONISHI Hiromitsu 大阪大学, 医学系・研究科, 助教 (20452435)
• Project Period (FY): 2009 – 2011
• Keywords: 核磁気共鳴画像(MRI) / 肝細胞特異性造影剤 / ガドキセト酸ナトリウム(Gd-EOB-DTPA) / 肝細胞 / organic anion transporting polypeptide(OATP) / 肝細胞がん / アフリカツメガエル卵母細胞

Research Abstract

The main purpose of the present study was to investigate the transporters that mediate the uptake of hepatocyte-specific magnetic resonance imaging contrast agent, gadoxetate disodium. Human organic anion transporting polypeptide(OATP) 2 and 8 have been proven to mediate the uptake of gadoxetate disodium using human cRNA-injected Xenopus laevis oocytes. The other purpose was to evaluate the accuracy of detection of hypervascular hepatocellular carcinoma by gadoxetate disodium-enhanced MR imaging. gadoxetate disodium-enhanced MR imaging revealed significant improvement in sensitivity and diagnostic accuracy for detection of hypervascular hepatocellular carcinoma.

Research Products

• [Journal Article] Hypervascular hepatocellular carcinomas : detection with gadoxetate disodium-enhanced MR imaging and multiphasic multidetector CT (2012)
  • Author(s): Onishi H, Kim T, Imai Y, Hori M, Nagano H, Nakaya Y, Tsuboyama T, Nakamoto A, Tatsumi M, Kumano S, Okada M, Takamura M, Wakasa K, Tomiyama N, Murakami T
  • Journal Title: European Radiology Volume: 22巻 Pages: 845-854
  • Peer Reviewed
• [Journal Article] 肝臓のEOB・プリモビスト造影MRI本検査を有効に活用するための理解すべき課題 (2010)
  • Author(s): 大西裕満, 金東石, 堀雅敏, 巽光朗, 坪山尚寛, 中本篤, 富山憲幸
  • Journal Title: 日獨医報 Volume: 55巻 Pages: 167-178
• [Presentation] Can Human Organic Anion Transporting Polypeptide Mediate the Uptake of Gadoxetate Disodium into Hepatocytes?、Radiological Society of North America(RSNA) (2011)
  • Author(s): Onishi H, Kim T, Hori M, Tatsumi M, Nakamoto A, Tsuboyama T, Tomiyama N
  • Organizer: 97th Scientific Assembly and Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-11-30
• [Presentation] ランチョンセミナーV肝臓MRI : CO-OPERATIONの功績肝細胞特異性MR造影剤EOB・プリモビストと薬物トランスポーター～造影剤の肝細胞取り込み機序について～ (2010)
  • Author(s): 大西裕満
  • Organizer: 第38回日本磁気共鳴医学会大会
  • Place of Presentation: 茨城県つくば市
  • Year and Date: 2010-10-01
• [Presentation] Gd-EOB-DTPAと薬物トランスポーター～MRIによる生体分子イメージングの可能性～ (2010)
  • Author(s): 大西裕満
  • Organizer: 大阪大学大学院医学系研究科放射線統合医学講座同門会講演会
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2010-04-24
• [Presentation] 多血性肝細胞癌におけるGd-EOB-DTPA造影ダイナミックMRIの診断能:造影ダイナミックCTとの比較 (2010)
  • Author(s): 大西裕満、村上卓道、今井康陽、金東石、堀雅敏、坪山尚寛、中本篤、中矢泰裕、高村学、熊野正士
  • Organizer: 第69回日本医学放射線学会学術集会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-04-10
• [Presentation] Gd-EOB-DTPAの肝細胞取り込みに関与するトランスポーターの同定:遺伝子導入卵母細胞を用いた検討 (2010)
  • Author(s): 大西裕満、金東石、堀雅敏、巽光朗、坪山尚寛、中本篤、大須賀慶悟、前田登、東原大樹、友田要
  • Organizer: 第69回日本医学放射線学会学術集会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2010-04-10
• [Presentation] Hypervascular Hepatocellular Carcinomas : Detection with Gd-EOB-DTPA-enhanced MR Imaging and Contrast-enhanced Dynamic MDCT、Radiological Society of North America(RSNA) (2009)
  • Author(s): Onishi H, Murakami T, Kim T, Hori M, Imai Y, Nagano H, Tsuboyama T, Nakaya Y, Nakamoto A, Kumano S, Takamura M
  • Organizer: 95th Scientific Assembly and Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-12-01
• [Book] 画像診断別冊KEY BOOKシリーズ肝胆膵の画像診断. CT・MRIを中心に (2010)
  • Author(s): 大西裕満, 他
  • Total Pages: 12(250-261)
  • Publisher: 秀潤社
• [Book] Diagnosis of Hepatocellular Carcinoma : Multidetector-Row Computed Tomography and Magnetic Resonance Imaging (2009)
  • Author(s): Onishi H, Murakami T, Nakamura H
  • Total Pages: 15(221-235)
  • Publisher: Springer