Role of inhibitory signal factors in osteoclastogenesis

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791411
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: HIKATA Tomohiro Keio University, 医学部, 研究員 (20348684)
• Project Period (FY): 2009 – 2010
• Keywords: 骨 / 軟骨代謝学

Research Abstract

Osteoclasts are unique bone-resorbing cells, and that loss of osteoclast differentiation or function results in defect of bone homeostasis. Thus regulation of osteoclastogenesis is crucial to maintain bone homeostasis. Osteoclast differentiation is regulated in a delicate balance between positive and negative regulators, however, the precise mechanism is still unclear. In this study, we characterized the inhibitory roles of PIAS1 in osteoclast differentiation.

Research Products

• [Journal Article] Receptor activator of NF-kappaB (RANK) ligand induces ectodomain shedding of RANK in murine RAW264.7 macrophages. (2010)
  • Author(s): Hakozaki A, Yoda M, Tohmonda T, Furukawa M, Hikata T, Uchikawa S, Takaishi H, Matsumoto M, Chiba K, Horiuchi K, Toyama Y.
  • Journal Title: J Immunol. 184 Pages: 2442-2448
  • Peer Reviewed
• [Journal Article] A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. (2009)
  • Author(s): Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H.
  • Journal Title: Dev Cell. 17 Pages: 836-848
  • Peer Reviewed
• [Journal Article] IL-27 abrogates receptor activator of NF-kappa B ligand-mediated osteoclastogenesis of human granulocyte-macrophage colony-forming unit cells through STAT1-dependent inhibition of c-Fos. (2009)
  • Author(s): Furukawa M, Takaishi H, Takito J, Yoda M, Sakai S, Hikata T, Hakozaki A, Uchikawa S, Matsumoto M, Chiba K, Kimura T, Okada Y, Matsuo K, Yoshida H, Toyama Y.
  • Journal Title: J Immunol. 183 Pages: 2397-2406
  • Peer Reviewed
• [Journal Article] PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts. (2009)
  • Author(s): Hikata T, Takaishi H, Takito J, Hakozaki A, Furukawa M, Uchikawa S, Kimura T, Okada Y, Matsumoto M, Yoshimura A, Nishimura R, Reddy SV, Asahara H, Toyama Y.
  • Journal Title: Blood. 113 Pages: 2202-2212
  • Peer Reviewed