2010 Fiscal Year Final Research Report

production of monoclonal antibodies against prostate cancer stem cells using differential dendritic cell immunization

Research Project

Project/Area Number	21791532
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Urology
Research Institution	Nagasaki University
Principal Investigator	TAMURA Takahiko Nagasaki University, 医歯薬学総合研究科, 助教 (00434035)
Project Period (FY)	2009 – 2010
Keywords	腫瘍学 / 抗体作製
Research Abstract	The regulatory dendritic cells expressing HA antigen were immunized into mice. The anti-HA antibody was less induced compared with normal dendritic cells. However, OVA protein immunization after HA immunization demonstrated anti-OVA antibody was similarly induced, suggesting differential dendritic cell immunization protocol worked properly. Dendritic cells were shown to phagocyte the membrane proteins extracted from cultured cells. These dendritic cells were immunized into mice. Anti-cell surface proteins of culture cells were produced, but it was less in the case of regulatory dendritic cells.

(4 results)