2010 Fiscal Year Final Research Report
Exploratory and functional analysis of new regulatory factor on energy metabolism
| Project/Area Number |
21880037
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
AKIEDA Sayaka University of Miyazaki, フロンティア科学実験総合センター, 助教 (20549076)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 脂肪組織 / エネルギー代謝調節 / 肥満 / 生理活性物質 |
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| Research Abstract |
High fat diet (HFD) is well known to be one of the contributing factors for the development of obesity. When rats were bred with 60% HFD, most of them show a state of increased body weight with high adiposity. However, we found that 10% rats bred with HFD demonstrate obesity-resistant [diet resistant (DR)] that their weighs are less than those of either diet-induced obesity (DIO) or control rats. We found that the mesenteric fat mass of DR rats was significantly less than that of DIO rats and similar to that of control rats, even though there were no differences in spontaneous activity, calorie intake, respiratory quotient and body temperature between DIO and DR rats. To examine the gene expressions specific for the mesenteric adipose tissues of DR rats, we performed DNA microarray analysis. A new bioactive peptide (gene X) and its receptor (gene Y) were found as specific expressed genes in three out of seven DR rats, and these genes were expressed in the macrophage of adipose tissue. We will examine the effects of co-culturing of rat primary mesenteric adipocytes with macrophages expressing gene X and gene Y on adipogenesis. Moreover, we are preparing the analysis in double (gene X and Y) knock-in Tg rats.
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[Journal Article] SIRT1 regulates thyroid-stimulating hormone release by enhancing PIP5Kγ activity through deacetylation of specific lysine residues in mammals.2010
Author(s)
Akieda-Asai S, Kahyo T, Zaima N, Yao I, Hatanaka T, Ikegami K, Iemura S, Sugiyama S, Yokozeki T, Eishi Y, Koike M, Ikeda K, Chiba T, Yamaza H, Shimokawa I, Song SY, Matsuno A, Mizutani A, Sawabe M, Chao MV, Tanaka M, Kanaho Y, Natsume T, Date Y, McBurney MW, Guarente L, Setou M
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Journal Title
PLoS One 5(7)
Pages: e11755
Peer Reviewed
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