2010 Fiscal Year Final Research Report
Research on mechanism of a novel signal polypeptide-induced innate immune activation.
Project/Area Number |
21890125
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Virology
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Research Institution | National Institute of Biomedical Innovation (2010) Osaka University (2009) |
Principal Investigator |
KOBIYAMA Kouji National Institute of Biomedical Innovation, 研究員 (50547700)
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Project Period (FY) |
2009 – 2010
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Keywords | アジュバント / I型IFN / 自然免疫 |
Research Abstract |
The aim of this research is to clarify mechanism of a novel signal polypeptide (N'-CARD-PTD)-induced innate immune activation and adjuvanticity of N'-CARD-PTD. Previous research we identified histone H2B is involved in dsDNA-mediated innate immune activation. Recently we synthesized the newly fusion protein, N'-CARD (nuclear localization signal of H2B fused CARD of IPS-1), activated nuclear DNA helicase II (NDH)-mediated innate immune responses. In this research, we clarify that protein transduction domain fused N'-CARD (N'-CARD-PTD) activated dendritic cells depend on type I IFN, but not TLRs. As a vaccine adjuvant, N'-CARD-PTD strongly induced antigen specific antibody response against various antigen.
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Research Products
(11 results)
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[Journal Article] Plasmacytoid dendritic cells delineate immunogenicity of influenza vaccine subtypes.2010
Author(s)
Koyama S, Aoshi T, Tanimoto T, Kumagai Y, Kobiyama K, Tougan T, Sakurai K, Coban C, Horii T, Akira S, Ishii KJ.
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Journal Title
Sci Transl Med. 2
Pages: 25ra24
Peer Reviewed
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[Journal Article] Extra-chromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010
Author(s)
Kobiyama K, Takeshita F, Jounai N, Sakaue-Sawano A, Miyawaki A, Ishii KJ, Kawai T, Sasaki S, Hirano H, Ishii N, Okuda K, Suzuki K.
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Journal Title
J.Virol. 84
Pages: 822-832
Peer Reviewed
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[Remarks] ホームページ等
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