2010 Fiscal Year Final Research Report
Ciliary localization of Inv regulates in vivo function
| Project/Area Number |
21890221
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
FUKUI Hajime Kyoto Prefectural University of Medicine, 医学研究科, 助教 (80551506)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ゼブラフィッシュ / 繊毛 |
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| Research Abstract |
We analyzed about the linkage between Inv function and cellular localization by using zebrafish. Inv has two calmodulin binding motifs (IQ domains), and we assessed about function of IQ domains. As a result, we found IQ domain deleted Inv could not localize to the cilium, and could not rescue the inv mutated phenotypes, including kidney cyst and left-right defect. In conclusion, cellular-ciliary localization of Inv was regulated by calmodulin binding, and it indicates that calmodulin dependent Inv localization requires in vivo functions.
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