Establishment of chorioallantoic membrane assay using oral cancer cells with high metastatic ability and identification of therapeutic target molecules

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K09862
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57020:Oral pathobiological science-related
• Research Institution: Fujita Health University
• Principal Investigator: Sakurai Kouhei 藤田医科大学, 医学部, 講師 (10608756)
• Co-Investigator(Kenkyū-buntansha): 酒井 康弘 藤田医科大学, 医学部, 講師 (20754394) ; 山田 勢至 藤田医科大学, 医学部, 准教授 (00566870) ; 林 雄一郎 横浜市立大学, 医学研究科, 客員研究員 (80806464)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 口腔癌 / 転移 / 鶏卵漿尿膜移植法 / 遺伝子発現

Outline of Final Research Achievements

Appropriate cancer cell models and animal experimental models that mimic cancer progression are required to analyze the metastasis of oral cancer cells, which occurs through complex steps. In this study, gene expression analysis was performed using HSC-3 cells and HSC-3-M3 cells and several genes related to prognosis were identified. In addition, chorioallantoic membrane assay was established as an animal experimental model. The tumors at the transplanted site could be histologically analyzed in detail using HE staining and immunohistochemical staining, and we have established the inexpensive and rapid analysis for gene expression and function.

Free Research Field

分子病理学

Academic Significance and Societal Importance of the Research Achievements

口腔癌の研究には様々な癌細胞株が利用されているが、各細胞株の特性・背景は異なっているため、解析結果を直接比較することが困難な場合も多い。本研究で用いたHSC-3細胞とHSC-3-M3細胞は同一患者の癌進展モデルとして有用と考えられた。また、本研究で確立した鶏卵漿尿膜移植法は、安価かつ迅速に癌進展を再現することができ、マウス実験に代わる（あるいはマウス実験の前段階で行う）動物実験と考えられた。