2023 Fiscal Year Final Research Report
Construction of eukaryotic cells lacking mitochondria and their molecular biological analysis
| Project/Area Number |
21K19204
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
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| Research Institution | Saitama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中井 由実 大阪医科薬科大学, 医学部, 講師 (80268193)
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| Project Period (FY) |
2021-07-09 – 2024-03-31
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| Keywords | 鉄硫黄クラスター / ミトコンドリア |
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| Outline of Final Research Achievements |
In the budding yeast Saccharomyces cerevisiae, the only essential function of mitochondria is the biosynthesis of iron-sulfur (Fe-S) clusters by the ISC machinery. In this study, we attempted to create “eukaryotic cells without mitochondria” by modifying yeast so that it can biosynthesize Fe-S clusters only in the cytosol. By expressing components of the prokaryotic SUF machinery in the cytosol, we succeeded for the first time in disrupting the NFS1 gene that encodes an essential component of ISC machinery. This gene disruption indicates that the essential function of the mitochondrial ISC machinery could be bypassed by the foreign SUF machinery expressed in the cytosol. Next, we attempted under similar conditions, to disrupt the gene for Tim23, a central component of the protein transport machinery from the cytosol to the mitochondria. To date, we have not succeeded in isolating the deletion strain, but we have paved the way for future challenges.
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| Free Research Field |
分子生物学、生化学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究においてNfs1を欠失できたことは、ミトコンドリアISCマシナリーの必須機能を、サイトゾルに導入した古細菌由来のSUFマシナリーによってバイパスさせることができたことを示している。引き続き、同様の条件下で、サイトゾルからミトコンドリアへのタンパク質輸送装置の中心成分であるTim23の遺伝子の破壊を試みたが、現在まで、破壊株の単離には成功していない。ただし、今後の挑戦に向けて道筋をつけることはできた。
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