2023 Fiscal Year Final Research Report

Investigation of the mechanism of nucleic acid metabolism to establish a a new therapeutic strategy.

Research Project

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Project/Area Number	21K19566
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
Research Institution	Kyoto University
Principal Investigator	Kitamura Sachiko 京都大学, 医学研究科, 助教 (10846140)
Co-Investigator(Kenkyū-buntansha)	三瀬名丹自治医科大学, 医学部, 准教授 (00360644) 山ノ井康二京都大学, 医学研究科, 助教 (70868075) 滝真奈京都大学, 医学研究科, 助教 (20898077)
Project Period (FY)	2021-07-09 – 2024-03-31
Keywords	卵巣癌 / 核酸 / dNTP
Outline of Final Research Achievements	We used our lab's qPCR equipment and collaborated with an experienced laboratory to attempt creating a calibration curve for dNTP. Although there were issues with reproducibility and it took longer than expected, we eventually managed to evaluate it semi-quantitatively. It was confirmed that the dTTP concentration in OVCAR5 and OVCAR3 cell lines decreased with the use of 5-FU. Measuring clinical samples is challenging, but this method is effective for understanding condition changes in vitro, and we plan to continue measuring dNTP. In exploring the world of metabolism, we discovered that changes in fatty acids inhibit cell growth, leading to conference presentations and paper publications. Having established the foundation for research using qPCR, we aim to further explore the impact of nucleic acid metabolism on gynecological malignancies and propose new therapeutic approaches.
Free Research Field	婦人科悪性腫瘍
Academic Significance and Societal Importance of the Research Achievements	dNTPの代謝動体の変化を詳細に探索する当初の研究目標は、期限内に達成することができなかったが、測定方法の基礎を築くことはできた。今後は、核酸代謝（dNTP）の動態評価を加えることが可能になったので、より深く代謝が受ける影響を探索することができると考えている。また、核酸ではなく脂肪という側面ではあるが、Oncogenic pathwayに直接影響を及ぼす薬剤を使わずとも、周囲からの代謝への影響で、がん細胞の増殖を抑制できうることも本研究の派生で示すことができた。今後も引き続き、核酸代謝が特に婦人科悪性腫瘍に及ぼす影響を探索し、新たな切り口からの治療法提案につなげたいと考えている。