2022 Fiscal Year Final Research Report
Analysis of common molecular mechanisms associated with epithelium differentiation and hair growth
| Project/Area Number |
21K19578
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
自見 英治郎 九州大学, 歯学研究院, 教授 (40276598)
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| Project Period (FY) |
2021-07-09 – 2023-03-31
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| Keywords | 転写共役因子 / 上皮細胞 / 間葉系細胞 / 上皮間葉相互作用 / Smad4 |
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| Outline of Final Research Achievements |
We established a mouse line of epithelium-specific conditional knockout of Smad4 by crossing Smad4 floxed mice with Keratin 14-Cre mice. These mice showed an abnormal tooth development phenotype, which is regulated by the epithelial-mesenchymal interaction. In RNA-seq analysis using control and Smad4 cKO mice, the expression levels of a gene involved in hair growth were changed in Smad4 cKO mice.
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| Free Research Field |
骨代謝学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、成長因子TGF-βファミリーの細胞内シグナルに必須の転写共役因子Smad4の機能を、マウスの上皮細胞特異的に解析できるコンディショナルノックアウトマウスが樹立できた。このマウスで認められた歯の形成不全は、歯の形成における上皮間葉相互作用において、TGF-βファミリーの重要性を示唆する。また、遺伝子発現の変化を解析した結果から、発毛に関わる遺伝子が見出されたことは、Smad4シグナルが上皮細胞における発毛に重要なことを示す。
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