A systems biology approach for improving baculovector system

2013 Fiscal Year Final Research Report

• Project/Area Number: 22248003
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Hokkaido University
• Principal Investigator: BANDO Hisanori 北海道大学, (連合)農学研究科(研究院), 教授 (20189731)
• Co-Investigator(Kenkyū-buntansha): KUSAKABE Takahiro 九州大学, 大学院農学研究院, 教授 (30253595) ; SATOU Masanao 基礎生物学研究所, 発生遺伝学研究部門, 助教 (20517693)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: バキュロウイルス / 遺伝子相互作用 / 昆虫利利用・機能開発

Research Abstract

By taking a systems biology approach, a baculovirus gene network models was built and host factors involved in recombinant protein expression from a baculovector system were identified.

Research Products

• [Journal Article] Construction and phenotypic analysis of BmNPVs lacking viral gene sequences. (2013)
  • Author(s): Hitomi Taka, Chikako Ono, Masanao Sato, Shin-ichiro Asano1 and Hisanori Bando
  • Journal Title: Journal of Insect Biotechnology and Sericology Volume: 82
  • Peer Reviewed
• [Journal Article] Phenotypic grouping of 141 BmNPVs lacking viral gene sequences. (2012)
  • Author(s): Chikako Ono, Takanori Kamagata, Hitomi Taka, Ken Sahara, Shin-ichiro Asano, Hisanori Bando
  • Journal Title: Virus Research Volume: 165 Pages: 197-206
  • Peer Reviewed
• [Presentation] 非必須遺伝子の複数ノックアウトがBmNPV増殖に与える影響について (2014)
  • Author(s): 高ひとみ、小野慎子、佐藤昌直、浅野眞一郎、伴戸久徳
  • Organizer: 日本蚕糸学会第83回蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 藤沢市
  • Year and Date: 20140310-11
• [Presentation] ウイルス遺伝子ネットワークモデリング:ウイルス学者は-omic から何を読み取るか (2013)
  • Author(s): 佐藤昌直
  • Organizer: 日本ウイルス学会北海道支部会 第47回夏季ンポジウム
  • Place of Presentation: 奈井江町(北海道)
  • Year and Date: 20130720-21
  • Invited
• [Presentation] Complex genetic interactions among non-essential genes of BmNPV revealed by multiple gene knockout analysis (2013)
  • Author(s): Hitomi Taka, Chikako Ono, Masanao Sato, Shin-ichiro Asano, Hisanori Bando
  • Organizer: International Conference on Agricultural Biodiversity and Sustainability
  • Place of Presentation: Daegu, Korea.
  • Year and Date: 20130617-24
• [Presentation] AcMNPVIE1 遺伝子発現のロバストネスを支えるIE遺伝子ネットワークと保証的相互作用 (2013)
  • Author(s): 小野慎子、高ひとみ、佐藤昌直、浅野眞一郎、伴戸久徳
  • Organizer: 日本蚕糸学会第84回大会 蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 藤沢市
  • Year and Date: 20130318-19
• [Presentation] 複数遺伝子ノックアウトウイルスを用いたウイルス遺伝子相互作用解析 (2013)
  • Author(s): 高ひとみ、小野慎子、佐藤昌直、浅野眞一郎、伴戸久徳
  • Organizer: 日本蚕糸学会第83回大会 蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: つくば市
  • Year and Date: 20130318-19
• [Presentation] Modeling the gene regulatory network of Bombyx mori nucleopolyhedrovirus T3 strain in early stage of the infection (2012)
  • Author(s): Masanao Sato
  • Organizer: Foundation of Systems Biology in Engineering 2012
  • Place of Presentation: Morioka, Japan
  • Year and Date: 20121021-25
• [Presentation] ノックアウトバキュロウイルスの作製と解析 (2012)
  • Author(s): 高ひとみ、小野慎子、浅野眞一郎、伴戸久徳
  • Organizer: 第10回昆虫病理研究会シンポジウム
  • Place of Presentation: 帯広市
  • Year and Date: 20120921-23
• [Presentation] Construction and phenotypic analysis of BmNPVs lacking viral gene sequences (2012)
  • Author(s): Hitomi Taka, Chikako Ono, Takanori Kamagata, Shin-ichiro Asano, Hisanori Bando
  • Organizer: International Conference on Agricultural Biodiversity and Sustainability
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20120728-29
• [Presentation] バキュロウイルス遺伝子発現制御機構の解明と利用に向けた基盤構築 (2012)
  • Author(s): 高ひとみ、伴戸久徳
  • Organizer: 日本ウイルス学会北海道支部会 第46回夏季シンポジウム
  • Place of Presentation: 伊達市
  • Year and Date: 20120721-22
• [Presentation] カイコBES を用いたヒト抗菌タンパク質の生産と活性評価 (2011)
  • Author(s): 永田祐大、副嶋泰彦、門宏明、李在萬、日下部宜宏
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 20111213-16
• [Presentation] カイコβ -N-acetyl glucosaminidase (BmFDL) のノックダウンがN- グリカン形成に与える影響 (2011)
  • Author(s): 永田祐大、副嶋泰彦、門宏明、李在萬、日下部宜宏
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 20111213-16
• [Presentation] バキュロウイルス発現系における小胞体シャペロンの関与 (2011)
  • Author(s): 工藤遼亮、門宏明、李在萬、日下部宜宏
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 20111213-16
• [Presentation] The replication properties of BmNPV controlled by a membrane fusion protein (2011)
  • Author(s): 酒井真美、佐原健、浅野眞一郎、伴戸久徳
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 20111213-16
• [Presentation] Analysis of a novel transactivator BmNPV p15 (2011)
  • Author(s): Yukimi Akatsuka, Takumi Abe, Yasuyuki Nishijima, Chikako Ono, Ken Sahara, Shin-ichiro Asano, Hisanori Bando
  • Organizer: XLIV Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: Halifax, Canada
  • Year and Date: 20110807-11
• [Presentation] hr5 as a cis-acting element escaping baculovirus -induced host shut-off (2010)
  • Author(s): Daisuke Ohtsuka, Sin-ichiro Asano, Ken Sahara, Hisanori Bando
  • Organizer: XLIII Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: Trabzon, Turkey
  • Year and Date: 20100711-15
• [Presentation] Essential genes of BmNPV (2010)
  • Author(s): Chikao Ono, Ken Sahara, Shn-ichiro Asano, Hisanori Bando
  • Organizer: XLIII Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: Trabzon, Turkey
  • Year and Date: 20100711-15
• [Remarks]
  • URL: http://jsss.agr.hokudai.ac.jp/BmNPVpro.htm