Identification of phospholipase activity of PRMT8 and its biological significance

2012 Fiscal Year Final Research Report

• Project/Area Number: 22248040
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: FUKAMIZU Akiyoshi 筑波大学, 生命環境系, 教授 (60199172)
• Project Period (FY): 2010 – 2012
• Keywords: アルギニンメチル化酵素 / メチル化アルギニン

Research Abstract

Among the family of protein arginine methyltransferases, PRMT1 accounts for over 85% of the total arginine methyltransferase activities in cells, and it is localized in the cytoplasm and nucleus. Although PRMT8 that has 83% amino acid homology to PRMT1, its biological significance and substrate remain unclear. In the present study, we tried to examine the enzymatic activity of PRMT8 and its cellular function.

Research Products

• [Journal Article] Enhanced histamine production through the induction of histidine decarboxylase expression by phorbol ester in Jurkat cells. (2012)
  • Author(s): Nagashima Y., Kako K., Kim JD., Fukamizu A.
  • Journal Title: Mol. Med. Rep. Volume: 6 Pages: 944-948
  • DOI: Doi:10.3892/mmr.2012.1049
  • Peer Reviewed
• [Journal Article] Production of free methylarginines via the proteasome and autophagy pathways in cultured cells. (2011)
  • Author(s): Shirakawa, T., Kako, K., Shimada, T.,Nagashima, Y., Nakamura, A., Ishida, J., Fukamizu, A.
  • Journal Title: Mol. Med. Rep Volume: 4 Pages: 615-620
  • DOI: Doi:10.3892/mmr.2011.488
  • Peer Reviewed
• [Journal Article] Asymmetric Arginine Dimethylation Determines Life Span in C. elegans by Regulating Forkhead Transcription Factor DAF-16. (2011)
  • Author(s): Takahashi, Y., Daitoku, H., Hirota, K., Tamiya, H., Yokoyama, A., Kako, K., Nagashima, Y., Nakamura, A., Shimada, T., Watanabe, S., Yamagata, K., Yasuda, K., Ishii, N., Fukamizu, A.
  • Journal Title: Cell Metab. Volume: 13 Pages: 505-516
  • DOI: Doi:10.1016/j.cmet.2011.03.017
  • Peer Reviewed
• [Journal Article] Arginine methylation of BCL-2 antagonist of cell death (BAD) counteracts its phosphorylation and inactivation by Akt. (2011)
  • Author(s): Sakamaki, JI., Daitoku, H., Ueno, K., Hagiwara, A., Yamagata, K., Fukamizu, A.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 15 Pages: 6085-6090
  • DOI: Doi:10.1073/pnas.1015328108
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of endothelial tip cell-enriched genes. (2010)
  • Author(s): Toro, R., Prahst, C., Mathivet, T., Siegfried, G., Kaminker, J., Larrivee, B., Breant, C., Duarte, A., Takakura, N., Fukamizu, A., Penninger, J., Eichmann, A.
  • Journal Title: Blood. Volume: 116 Pages: 4025-4033
  • DOI: Doi:10.1182/blood-2010-02-270819
  • Peer Reviewed
• [Presentation] Jurkat細胞におけるホルボールエステル刺激下でのヒスチジン脱炭酸酵素誘導性ヒスタ ミンの直接測定 (2012)
  • Author(s): 永島裕介、加香孝一郎、金 俊達、深水昭吉
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] Jurkat細胞におけるホルボールエステル刺激下でのヒスチジン脱炭酸酵素誘導性ヒスタ ミンの直接測定 (2012)
  • Author(s): 永島裕介、加香孝一郎、金 俊達、深水昭吉
  • Organizer: 第7回日本ケミカルバイオロジー学会年会
  • Place of Presentation: 京都大学
  • Year and Date: 2012-06-08
• [Presentation] 1mmの生物が教えてくれる病気と寿命のメカニズム (2011)
  • Author(s): 深水昭吉
  • Organizer: 第14回山口大学イブンニングセミナー2010
  • Place of Presentation: 東京・キャンパス・イノベーションセンター
  • Year and Date: 2011-02-10
• [Presentation] Quantitative analysis of free methylarginines and biosynthesis via protein degradation in cultured cells. (2010)
  • Author(s): Shirakawa, T., Kako, K., Nagashima, Y., Nakamura, A., Shimada, T., Fukamizu, A.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Arginine methylation of BAD counteracts its phosphorylation and inactivation by AKT. (2010)
  • Author(s): Sakamaki, J., Daitoku, H., Ueno, K., Yamagata, K., Fukamizu, A.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Decoding of Forkhead Transcription Factors' Function by Arginine Methylation and PKB/Akt-Induced Phosphorylation. (2010)
  • Author(s): Fukamizu, A.
  • Organizer: The 1^<st> International Symposium on Brain Function and Disorders (SBFD)
  • Place of Presentation: 中国・北京
  • Year and Date: 2010-10-25
• [Presentation] Cross-talk regulation of FOXO transcription factor by arginine methylation and PKB/Akt-induced Phosphorylation. (2010)
  • Author(s): Fukamizu, A.
  • Organizer: 15th World Congress on Advances in Oncology and 13th International Symposium on Molecular Medicine
  • Place of Presentation: ギリシャ・アテネ
  • Year and Date: 2010-10-09
• [Presentation] Arginine methylation controls lifespan in C. elegans. (2010)
  • Author(s): Takahashi, Y., Daitoku, H., Yamagata, K., Hirota, K., Yasuda, K., Ishii, N., Fukamizu, A.
  • Organizer: 4^<th> East Asia C.elegans Meeting
  • Place of Presentation: 東京・国立オリンピック記念青少年総合センター
  • Year and Date: 2010-07-12
• [Presentation] Methylation of arginine decodes protein functions regulated by PKB/Akt-induced phosphorylation. (2010)
  • Author(s): Fukamizu, A.
  • Organizer: 2010 FASEB Summer Research Conferences
  • Place of Presentation: アメリカ合衆国・アリゾナ
  • Year and Date: 2010-06-09
• [Presentation] Arginine methylation of BAD counteracts its phosphorylation and inactivation by AKT. (2010)
  • Author(s): Sakamaki, J., Daitoku, H., Ueno, K., Yamagata, K., Fukamizu, A.
  • Organizer: 2010 FASEB Summer Research Conferences
  • Place of Presentation: アメリカ合衆国・アリゾナ
  • Year and Date: 2010-06-09
• [Presentation] Arginine methylation controls lifespan in C. elegans. (2010)
  • Author(s): Takahashi, Y., Daitoku, H., Yamagata, K., Hirota, K., Yasuda, K., Ishii, N., Fukamizu, A.
  • Organizer: 2010 FASEB Summer Research Conferences
  • Place of Presentation: アメリカ合衆国・アリゾナ
  • Year and Date: 2010-06-09