2012 Fiscal Year Final Research Report
Visualization analysis of neurotransmission and exocytosis by using novel fluorescent protein and laser optics technology
| Project/Area Number |
22300131
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fusional basic brain science
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| Research Institution | Hokkaido University |
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Principal Investigator |
NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 2光子顕微鏡 / 分泌 / 蛍光タンパク質 / SNARE / 光脳科学 / 非線形光学 |
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| Research Abstract |
We have developed novel visualization analysis techniques for neurotransmission and exocytosis, and constructed “in vivo” optical imaging methods to advance for elucidation of the molecular basis. We generated fluorescently tagged SNARE proteins, VAMP-Sirius by using a novel fluorescent protein Sirius in order to visualize dynamics of acid vesicles in an osteoclast model. A novel FRET Ca^<2+> sensor, Cameleon-nano protein, visualized Ca^<2+>-dependent exocyosis in pancreatic acinar cells, as well as Ca^<2+> dynamics in neurons in cerebellum. Furthermore, we successfully observed hippocampal neurons in deeper layers in live mouse brains.
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[Journal Article] Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb2011
Author(s)
Masato Sawada, Naoko Kaneko, HiroyukiInada, Hiroaki Wake, Yasuko Kato, Yuchio Yanagawa, Kazuto Kobayashi, Tomomi Nemoto, Junichi Nabekura, and Kazunobu Sawamoto, J
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Journal Title
Neurosci
Volume: vol.31
Pages: 11587-11596
DOI
Peer Reviewed
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[Journal Article] Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in a RAPL-dependent and-independent manner2010
Author(s)
Yukihiko Ebisuno, Koko Katagiri, Tomoya Katakai, Yoshihiro Ueda, Tomomi Nemoto, Hiroyuki Inada, Junichi Nabekura, Takaharu Okada, Reiji Kannagi, Toshiyuki Tanaka, Masayuki Miyasaka, Nancy Hogg, Tatsuo Kinashi
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Journal Title
Blood
Volume: 115(4)
Pages: 804-14
DOI
Peer Reviewed
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[Presentation] In vivo two-photon microscopy with a 1030 nm (high-peak-power) picosecond-pulse laser to visualize the cortexand hippocampal pyramidal neurons in H-Line mice2012
Author(s)
R. Kawakami, K. Sawada, A. Sato, T. Hibi, Y.Kozawa, S. Sato, H. Yokoyama, T. Nemoto
Organizer
Society for Neuroscience
Place of Presentation
Ernest N. Morial Convention Center, New Orleans, US, 2012
Year and Date
20121013-17
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[Presentation] Improvement of the Spatial Resolution in Two-Photon Microscopy with a Vector Beam Generated by Liquid Crystal Devices2012
Author(s)
S. Ipponjima, T. Terumasa, Y. Kozawa , H.Horanai, A. Sato, M. Kurihara, N. Hashimoto, H. Yokoyama, S. Sato, T. Nemoto
Organizer
Focus On Microscopy 2012, Suntec Singapore International Convention & Exhibition centre
Place of Presentation
Singapore, Republic of Singapore
Year and Date
20120401-04
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