2012 Fiscal Year Final Research Report
Development of autoimmune mouse model by viral factors
| Project/Area Number |
22300140
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
|
| Keywords | パルボウイルス / NS / Bmper / DNA メチル化 / コラーゲン関節炎 / マウス |
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| Research Abstract |
In the present study, it was elucidated that parvoviral nonstructure (NS) protein induced DNA methylation in T lymphocytes and caused change of apoptosis resistancy and cell proliferation. Moreover, a possible effect of NS for incidence of collagen-induced arthritis was examined in vivo. The most of mouse T lymphocytes infected with NS-EGFP expression vector caused apoptosis, and the survived cells suppressed Bmper expression by DNA methylation. These cells acquired to be resistant for parvoviral re-infection. DBA/1 mice infected with NS-EGFP expression vector developed collagen-induced arthritis, though there was no significant difference on incidence of them to that of the control.
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