Creation of Functional Artificial Peptide Nanocapsules based onInternal Skeleton of Viral Capsids

2012 Fiscal Year Final Research Report

• Project/Area Number: 22350075
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Tottori University (2012); Kyushu University (2010-2011)
• Principal Investigator: MATSUURA Kazunori 鳥取大学, 大学院・工学研究科, 教授 (60283389)
• Project Period (FY): 2010 – 2012
• Keywords: ペプチド / 自己集合 / ウイルスキャプシド / ナノカプセル

Research Abstract

Recently, application of plant viruses to nanotechnology have attracted much attention due to their fascinating nanostructures with discrete nano-space. However, it remains difficult to rationally design artificial viral capsid. In this project, we found that a synthesized viral β-annulus peptide (24 residues) showed spontaneous self-assembly into hollow nanocapsules (synthetic viral capsid) with the size of 30-50 nm in water. Moreover, we have succeeded in synthesizing magnetite and encapsulating M13 phage DNA into the artificial capsids.

Research Products

• [Journal Article] (2013)
  • Author(s): K. Matsuura, K. Watanabe, Y. Matsushita and N. Kimizuka
  • Journal Title: Polymer J Volume: 45 Pages: 529-534
  • DOI: DOI: 10.1038/pj.2012.235
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): K. Matsuura
  • Journal Title: Polymer J Volume: 44 Pages: 469-474
  • DOI: DOI: 10.1038/pj.2012.16
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): K. Matsuura, K. Tochio, K. Watanabe, and N. Kimizuka
  • Journal Title: Chem. Lett Volume: 7 Pages: 711-713
  • DOI: DOI: 10.1246/cl.2011.711
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): K. Matsuura, H. Hayashi, K. Murasato, and N. Kimizuka
  • Journal Title: Chem. Commun Volume: 47 Pages: 265-267
  • DOI: DOI: 10.1039/C0CC01324B
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): K. Matsuura, K. Watanabe, K. Sakurai, T. Matsuzaki, and N. Kimizuka
  • Journal Title: Angew. Chem. Int. Ed Volume: 49 Pages: 9662-9665
  • DOI: DOI: 10.1002/anie.201004606
  • Peer Reviewed
• [Presentation] K. Matsuura(2) (2012)
  • Author(s): K. Matsuura
  • Organizer: Nanopeptide 2012: Peptides as Nanomaterials & Biomaterials
  • Place of Presentation: University of Manchester(英国)
  • Year and Date: 2012-11-14
• [Presentation] Peptide Nanocapsule Self-assembled from Viral β-Annulus Peptide (2011)
  • Author(s): K. Matsuura 他 3 名
  • Organizer: The 3rd Asian Symposium on Advanced Materials
  • Place of Presentation: 九州大学
  • Year and Date: 2011-09-22
• [Presentation] ウイルス由来ペプチドの自己集合によるナノカプセルの創製 (2011)
  • Author(s): 松浦和則
  • Organizer: 第27回日本DDS学会学術集会
  • Place of Presentation: 東京大学
  • Year and Date: 2011-06-19
• [Presentation] Creation of Peptide Nanocapsules based on Viral Self-assembly (2010)
  • Author(s): K. Matsuura
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: Hawaii Convention Center(米国)
  • Year and Date: 2010-12-18
• [Presentation] ウイルスの構築原理に基づくペプチドナノカプセルの創成 (2010)
  • Author(s): 松浦和則
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 第59回高分子学会年次大会
  • Year and Date: 2010-05-28
• [Book] ドラッグデリバリーシステムの新展開II-核酸医薬・抗体医薬・ワクチン医療を支えるDDS技術- (2012)
  • Author(s): 松浦和則 他
  • Total Pages: 231-234
  • Publisher: シーエムシー出版
• [Remarks] 2010/11/17 日経産業新聞
• [Remarks] ホームページ(Nature Japan特集記事, 2011年1月13日)
  • URL: http://www.natureasia.com/ja-jp/jobs/tokushu/detail/223
• [Remarks] 研究成果が以下のメディアに掲載された 科研費NEWSレター 2013年, Vol.1, 山陰中央新報 2013/3/26, 産経新聞夕刊 2013/3/25, 日本経済新聞夕刊 2013/3/25, 日本海新聞 2013/2/6, Yahooニュース 2013/1/25, マイナビニュース 2013/1/25, 産経新聞 2010/11/14, 西日本新聞夕刊 2010/11/13, Yahooニュース 2010/11/13