2012 Fiscal Year Final Research Report
Experimental Investigation of Response of Endothelial Cells andMeasurement of Fluid Shear Stress Using Blood Vessel Model
| Project/Area Number |
22360082
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fluid engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUGII Yasuhiko 東京大学, 大学院・工学系研究科, 特任准教授 (90345108)
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| Co-Investigator(Kenkyū-buntansha) |
HISHIDA Koichi 慶應義塾大学, 理工学部, 教授 (40156592)
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| Project Period (FY) |
2010 – 2012
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| Keywords | バイオ流体力学 / マイクロ流体 / 血管内皮細胞 / せん断応力 / 循環器病 |
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| Research Abstract |
To investigate an influence of a glycocalyx surface layer covering over endothelial cells (ECs) on the flow field or flow through the layer, a high spatial resolution measurement technique was improved for three-dimensional velocity distributions close to a glycocalyx layer by utilizing a confocal micro-PIV (Particle Image Velocimetry) technique. The developed technique achieved to measure three-dimensional velocity distributions in a177 × 177 × 3 m3 region with in-plane resolution of 7 m and with depth resolution of 0.5 m. By optimizing the PIV analysis, the measurement accuracy was improved even with small fluorescent particle of the diameter of 200 nm and large shear rate. Three-dimensional velocity distribution close to the layer of living ECs cultured in microchannel was measured. The results suggested that the developed technique was useful for investigation of velocity field close to the layer.
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