2014 Fiscal Year Final Research Report
Genetic system for functioning to prevent aging
| Project/Area Number |
22370003
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Yasumitsu 福岡歯科大学, 歯学部, 准教授 (20212003)
ITO Riyoko 福岡歯科大学, 口腔歯学部, 講師 (10140865)
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Keywords | RNA / 前駆体ヌクレオチド / 老化 / 発現異常 / 酸化ストレス / 分解酵素 / RNA結合タンパク質 / 8-オキソグアニン |
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| Outline of Final Research Achievements |
In aerobically growing cells, in which reactive oxygen species are produced, the guanine base of RNA is oxidized to 8-oxo-7,8-dihydroguanine, which induces alterations in gene expression. Human cells contain three types of enzymes, MTH1, MTH2 and MTH3, that degrade 8-oxoguanine-containing nucleotides. In addition, the human Auf1 protein, also called HNRNPD, binds specifically to RNA containing this oxidized base and may be involved in cellular processes associated with the problems caused by RNA oxidation. Auf-deficient cells were constructed from human HeLa and Nalm-6 lines using two different targeting procedures. Both types of Auf1-deficient cells are viable, but exhibit growth retardation. The level of oxidized messenger RNA was considerably higher in Auf1-deficient cells. Auf1 may play a role in the elimination of oxidized RNA, which is required for the maintenance of proper gene expression under oxidative stress.
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| Free Research Field |
生物学
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