2012 Fiscal Year Final Research Report
Protein interaction analysis of circadian clock protein
| Project/Area Number |
22370037
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKATSU Toru 京都大学, 大学院・薬学研究科, 准教授 (50293949)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 生体リズム |
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| Research Abstract |
BMAL1 and CLOCK are the transcription factors of the circadian clock in mammals. The human BMAL1:CLOCK bHLH domain forms hetero or homo dimer and binds to E-box DNA. The hetero dimer of bHLH domain was more preferable to recognize the E-BOX DNA compared to the homo dimer based on the ITC analysis. Next we investigated the region related to the formation of the bHLH PAS-A domain of the human BMAL1:CLOCK hetero dimer. It is suggested that the bHLH domain is more important for the hetero dimer formation than the PAS-A domain.
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